| Autor: |
Ucieklak K; Hirszfeld Institute of Immunology and Experimental Therapy, 53-114 Wroclaw, Poland., Koj S; Hirszfeld Institute of Immunology and Experimental Therapy, 53-114 Wroclaw, Poland., Niedziela T; Hirszfeld Institute of Immunology and Experimental Therapy, 53-114 Wroclaw, Poland. |
| Jazyk: |
angličtina |
| Zdroj: |
International journal of molecular sciences [Int J Mol Sci] 2020 Sep 03; Vol. 21 (17). Date of Electronic Publication: 2020 Sep 03. |
| DOI: |
10.3390/ijms21176433 |
| Abstrakt: |
Whooping cough is a highly contagious disease caused predominantly by Bordetella pertussis , but it also comprises of a pertussis-like illness caused by B. holmesii . The virulence factors of B. holmesii and their role in the pathogenesis remain unknown. Lipopolysaccharide is the main surface antigen of all Bordetellae . Data on the structural features of the lipopolysaccharide (LPS) of B. holmesii are scarce. The poly- and oligosaccharide components released by mild acidic hydrolysis of the LPS were separated and investigated by 1 H and 13 C NMR spectroscopy, mass spectrometry, and chemical methods. The structures of the O-specific polysaccharide and the core oligosaccharide of B. holmesii ATCC 51541 have been identified for the first time. The novel pentasaccharide repeating unit of the B. holmesii O-specific polysaccharide has the following structure: {→2)-α-l-Rha p -(1→6)-α-d-Glc p -(1→4)-[β-d-Glc p NAc-(1→3]-α-d-Gal p -(1→3)-α-d-Glc p NAc-(1→} n . The SDS-PAGE and serological cross-reactivities of the B. holmesii LPS suggested the similarity between the core oligosaccharides of B. holmesii ATCC 51541 and B. pertussis strain 606. The main oligosaccharide fraction contained a nonasaccharide. The comparative analysis of the NMR spectra of B. holmesii core oligosaccharide fraction with this of the B. pertussis strain 606 indicated that the investigated core oligosaccharides were identical. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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