Gamma-Linolenic and Pinolenic Acids Exert Anti-Inflammatory Effects in Cultured Human Endothelial Cells Through Their Elongation Products.

Autor: Baker EJ; Faculty of Medicine, School of Human Development and Health, University of Southampton, Southampton, SO16 6YD, UK., Valenzuela CA; Faculty of Medicine, School of Human Development and Health, University of Southampton, Southampton, SO16 6YD, UK.; Faculty of Pharmacy, School of Nutrition, University of Valparaíso, Playa Ancha, 850, Valparaíso, Chile., van Dooremalen WTM; Faculty of Medicine, School of Human Development and Health, University of Southampton, Southampton, SO16 6YD, UK., Martínez-Fernández L; Faculty of Medicine, School of Human Development and Health, University of Southampton, Southampton, SO16 6YD, UK.; Department of Nutrition, Food Science and Physiology, School of Pharmacy and Nutrition, University of Navarra, 31008, Pamplona, Spain., Yaqoob P; School of Chemistry, Food and Pharmacy, University of Reading, Reading, RG6 6AP, UK., Miles EA; Faculty of Medicine, School of Human Development and Health, University of Southampton, Southampton, SO16 6YD, UK., Calder PC; Faculty of Medicine, School of Human Development and Health, University of Southampton, Southampton, SO16 6YD, UK.; NIHR Southampton Biomedical Research Centre, University Hospital Southampton NHS Foundation Trust and University of Southampton, Southampton, SO16 6YD, UK.
Jazyk: angličtina
Zdroj: Molecular nutrition & food research [Mol Nutr Food Res] 2020 Oct; Vol. 64 (20), pp. e2000382. Date of Electronic Publication: 2020 Sep 16.
DOI: 10.1002/mnfr.202000382
Abstrakt: Scope: Omega-3 fatty acids (FAs) from oily fish reduce cardiovascular disease. This may be partly due to modulation of endothelial cell (EC) inflammation. Fish stocks are declining and there is a need for sustainable alternative FAs. Gamma-linolenic acid (GLA) and pinolenic acid (PLA) are plant-derived FAs, which can fulfil this role.
Methods and Results: EA.hy926 cells are exposed GLA and PLA prior to stimulation with tumor necrosis factor (TNF)-α. GLA and PLA are incorporated into ECs, resulting in increases in long-chain derivatives produced by elongase 5, dihomo-gamma-linolenic acid (DGLA), and eicosatrienoic acid (ETA). Both GLA and PLA (50 µm) decrease production of soluble intercellular adhesion molecule-1 (sICAM-1), monocyte chemoattractant protein 1 (MCP-1), and regulated on activation, normal T cell expressed and secreted (RANTES). However, decreases in these mediators are not seen after pre-treatment with GLA or PLA in elongase 5 silenced EA.hy926 cells. DGLA and ETA (10 µm) decrease EC production of sICAM-1, MCP-1, RANTES, and IL-6. All FAs reduce adhesion of THP-1 monocytes to EA.hy926 cells. Both PLA (50 µm) and ETA (10 µm) decrease NFκBp65 phosphorylation.
Conclusion: These effects suggest potential for GLA, PLA and their long-chain derivatives, DGLA and ETA, as sustainable anti-inflammatory alternatives to fish-derived FAs.
(© 2020 The Authors. Published by Wiley-VCH GmbH.)
Databáze: MEDLINE
Externí odkaz: