| Autor: |
Shkurnikov MY; Hertsen Moscow Oncology Research Center, Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation, Moscow, Russia. mshkurnikov@gmail.com., Nersisyan SA; Faculty of Biology and Biotechnology, National Research University Higher School of Economics, Moscow, Russia.; Faculty of Mechanics and Mathematics, Moscow State University, Moscow, Russia., Osepyan AS; Faculty of Mechanics and Mathematics, Moscow State University, Moscow, Russia., Maltseva DV; Faculty of Biology and Biotechnology, National Research University Higher School of Economics, Moscow, Russia.; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia., Knyazev EN; Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia. knyazevevg@gmail.com. |
| Abstrakt: |
Human colorectal adenocarcinoma cell line Caco-2 is often used as a model of healthy intestinal epithelium, in particular, in miRNA studies. The work of the enzymes Drosha and Dicer is an integral part of the process of miRNA formation. Inaccuracies in the work of these enzymes lead to a change in the nucleotide sequences of miRNAs with the formation of new isoforms, which, in turn, can change intracellular regulatory mechanisms. In the framework of this study, it was shown that the quantitative estimates of inaccuracies in Drosha and Dicer activity significantly differ between the specimens of normal colon tissue and malignant colorectal tumors. |
