Metabolite profiling reveals the interaction of chitin-glucan with the gut microbiota.

Autor: Rodriguez J; Metabolism and Nutrition Research Group, Louvain Drug Research Institute, UCLouvain, Université Catholique de Louvain , Brussels, Belgium., Neyrinck AM; Metabolism and Nutrition Research Group, Louvain Drug Research Institute, UCLouvain, Université Catholique de Louvain , Brussels, Belgium., Zhang Z; Department of Medicine, University of Alberta , Edmonton, Canada., Seethaler B; Institute of Nutritional Medicine, University of Hohenheim , Stuttgart, Germany., Nazare JA; Rhône-Alpes Research Center for Human Nutrition, Université-Lyon, CarMeN Laboratory, Hospices Civils de Lyon , Lyon, France., Robles Sánchez C; Metabolism and Nutrition Research Group, Louvain Drug Research Institute, UCLouvain, Université Catholique de Louvain , Brussels, Belgium., Roumain M; Bioanalysis and Pharmacology of Bioactive Lipids Research Group, Louvain Drug Research Institute, UCLouvain, Université Catholique de Louvain , Brussels, Belgium., Muccioli GG; Bioanalysis and Pharmacology of Bioactive Lipids Research Group, Louvain Drug Research Institute, UCLouvain, Université Catholique de Louvain , Brussels, Belgium., Bindels LB; Metabolism and Nutrition Research Group, Louvain Drug Research Institute, UCLouvain, Université Catholique de Louvain , Brussels, Belgium., Cani PD; Metabolism and Nutrition Research Group, Louvain Drug Research Institute, UCLouvain, Université Catholique de Louvain , Brussels, Belgium.; WELBIO- Walloon Excellence in Life Sciences and BIOtechnology, UCLouvain, Université Catholique de Louvain , Brussels, Belgium., Maquet V; KitoZyme, Parc Industriel des Hauts-Sart , Herstal, Belgium., Laville M; Rhône-Alpes Research Center for Human Nutrition, Université-Lyon, CarMeN Laboratory, Hospices Civils de Lyon , Lyon, France., Bischoff SC; Institute of Nutritional Medicine, University of Hohenheim , Stuttgart, Germany., Walter J; Department of Agricultural, Food & Nutritional Science and Department of Biological Sciences, University of Alberta , Edmonton, Canada.; APC Microbiome Ireland, Department of Medicine, and School of Microbiology, University College Cork , Cork, Ireland., Delzenne NM; Metabolism and Nutrition Research Group, Louvain Drug Research Institute, UCLouvain, Université Catholique de Louvain , Brussels, Belgium.
Jazyk: angličtina
Zdroj: Gut microbes [Gut Microbes] 2020 Nov 09; Vol. 12 (1), pp. 1810530.
DOI: 10.1080/19490976.2020.1810530
Abstrakt: Dietary fibers are considered beneficial nutrients for health. Current data suggest that their interaction with the gut microbiota largely contributes to their physiological effects. In this context, chitin-glucan (CG) improves metabolic disorders associated with obesity in mice, but its effect on gut microbiota has never been evaluated in humans. This study explores the effect of a 3-week intervention with CG supplementation in healthy individuals on gut microbiota composition and bacterial metabolites. CG was given to healthy volunteers (n = 15) for three weeks as a supplement (4.5 g/day). Food diary, visual analog and Bristol stool form scales and a "quality of life" survey were analyzed. Among gut microbiota-derived metabolites, bile acids (BA), long- and short-chain fatty acids (LCFA, SCFA) profiling were assessed in stool samples. The gut microbiota (primary outcome) was analyzed by Illumina sequencing. A 3-week supplementation with CG is well tolerated in healthy humans. CG induces specific changes in the gut microbiota composition, with Eubacterium, Dorea and Roseburia genera showing the strongest regulation. In addition, CG increased bacterial metabolites in feces including butyric, iso-valeric, caproic and vaccenic acids. No major changes were observed for the fecal BA profile following CG intervention. In summary, our work reveals new potential bacterial genera and gut microbiota-derived metabolites characterizing the interaction between an insoluble dietary fiber -CG- and the gut microbiota.
Databáze: MEDLINE
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