Analysis of chicken intestinal natural killer cells, a major IEL subset during embryonic and early life.

Autor: Meijerink N; Department Biomolecular Health Sciences, Division Infectious Diseases and Immunology, Yalelaan 1, 3584, CL, the Netherlands. Electronic address: n.meijerink@uu.nl., van Haarlem DA; Department Biomolecular Health Sciences, Division Infectious Diseases and Immunology, Yalelaan 1, 3584, CL, the Netherlands. Electronic address: d.a.vanhaarlem@uu.nl., Velkers FC; Department Population Health Sciences, Division Farm Animal Health, Yalelaan 7, 3584, CL, the Netherlands; Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands. Electronic address: f.c.velkers@uu.nl., Stegeman AJ; Department Population Health Sciences, Division Farm Animal Health, Yalelaan 7, 3584, CL, the Netherlands; Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands. Electronic address: j.a.stegeman@uu.nl., Rutten VPMG; Department Biomolecular Health Sciences, Division Infectious Diseases and Immunology, Yalelaan 1, 3584, CL, the Netherlands; Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Pretoria, South Africa. Electronic address: v.rutten@uu.nl., Jansen CA; Department Biomolecular Health Sciences, Division Infectious Diseases and Immunology, Yalelaan 1, 3584, CL, the Netherlands. Electronic address: c.a.jansen@uu.nl.
Jazyk: angličtina
Zdroj: Developmental and comparative immunology [Dev Comp Immunol] 2021 Jan; Vol. 114, pp. 103857. Date of Electronic Publication: 2020 Sep 04.
DOI: 10.1016/j.dci.2020.103857
Abstrakt: Restrictions on antimicrobials demand alternative strategies to improve broiler health, such as supplying feed additives which stimulate innate immune cells like natural killer (NK) cells. The main objective of this study was to characterize intestinal NK cells in broiler chickens during embryonic and early life and compare these to NK cells in spleen, blood and bone marrow. Also T-cell subsets were determined. The majority of intestinal NK cells expressed IL-2Rα rather than 20E5 and 5C7, and showed low level of activation. Within intestinal NK cells the activation marker CD107 was mostly expressed on IL-2Rα + cells while in spleen and blood 20E5 + NK cells primarily expressed CD107. High percentages of intestinal CD8αα + , CD8αβ + and from 2 weeks onward also gamma delta T cells were found. Taken together, we observed several intestinal NK subsets in broiler chickens. Differences in NK subsets were mostly observed between organs, rather than differences over time. Targeting these intestinal NK subsets may be a strategy to improve immune-mediated resistance in broiler chickens.
(Copyright © 2020 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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