Magnetic particle transport through organogel - an application to DNA extraction.

Autor: Ohashi T; Technology Research Laboratory, Shimadzu Corporation, 3-9-4 Hikaridai, Seika-cho, Soraku-gun, Kyoto, 619-0237, Japan. Electronic address: tetu@shimadzu.co.jp., Kuyama H; Technology Research Laboratory, Shimadzu Corporation, 3-9-4 Hikaridai, Seika-cho, Soraku-gun, Kyoto, 619-0237, Japan.
Jazyk: angličtina
Zdroj: Analytical biochemistry [Anal Biochem] 2020 Dec 15; Vol. 611, pp. 113932. Date of Electronic Publication: 2020 Sep 04.
DOI: 10.1016/j.ab.2020.113932
Abstrakt: An observation that magnetic particles are transported through organogel encouraged us to investigate its feasibility of liquid-phase displacement in DNA extraction using magnetic particles. Organogel for this study was prepared from a gelator, 12-hydroxystearic acid (12-HSA), and an apolar solvent, methylphenylsilicone oil. The organogel is a gel-like solid material with hydrophobic and elastic properties. These properties, hydrophobicity, and elasticity were demonstrated to be advantageous for liquid compartmentalization and efficient liquid-phase displacement. The extracted DNA with using the organogel device was successfully detected off-chip by conventional real-time PCR.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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