Jabuticaba peel extract modulates adipocyte and osteoblast differentiation of MSCs from healthy and osteoporotic rats.

Autor: Souza ATP; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Av Do Café S/N, Ribeirão Preto, SP, 14040-904, Brazil., Freitas GP; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Av Do Café S/N, Ribeirão Preto, SP, 14040-904, Brazil., Lopes HB; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Av Do Café S/N, Ribeirão Preto, SP, 14040-904, Brazil., Totoli GGC; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Av Do Café S/N, Ribeirão Preto, SP, 14040-904, Brazil., Tarone AG; School of Food Engineering, University of Campinas, Rua Monteiro Lobato 80, Campinas, 13083-862, SP, Brazil., Marostica-Junior MR; School of Food Engineering, University of Campinas, Rua Monteiro Lobato 80, Campinas, 13083-862, SP, Brazil., Rosa AL; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Av Do Café S/N, Ribeirão Preto, SP, 14040-904, Brazil., Beloti MM; Bone Research Lab, School of Dentistry of Ribeirão Preto, University of São Paulo, Av Do Café S/N, Ribeirão Preto, SP, 14040-904, Brazil. mmbeloti@usp.br.
Jazyk: angličtina
Zdroj: Journal of bone and mineral metabolism [J Bone Miner Metab] 2021 Mar; Vol. 39 (2), pp. 163-173. Date of Electronic Publication: 2020 Sep 05.
DOI: 10.1007/s00774-020-01152-8
Abstrakt: Introduction: The jabuticaba peel extract (JPE) contains bioactive compounds that regulate fat metabolism. Because the negative correlation between fat accumulation and bone formation in bone marrow, we hypothesized that JPE inhibits adipocyte as well as favors osteoblast differentiation of mesenchymal stromal cells (MSCs) under healthy and osteoporotic conditions, a disease that display an imbalance between adipocyte and osteoblast differentiation resulting in reduced bone mass.
Material and Methods: To test these hypotheses, bone marrow MSCs were harvested from healthy and osteoporotic rats and cultured in adipogenic and osteogenic media with three concentrations of JPE, 0.25, 5 and 10 µg/ml, and vehicle (control). After selecting the most efficient concentrations of JPE, we used them to evaluate adipocyte and osteoblast differentiation of MSCs from both sources.
Results: We observed that, in general, JPE inhibited adipocyte differentiation of MSCs with more pronounced effects in cells from healthy than osteoporotic rats. In addition, JPE increased osteoblast differentiation, exhibiting a slightly higher osteogenic potential on MSCs from osteoporotic compared to healthy condition.
Conclusion: Our results demonstrated that JPE drives MSCs to inhibit adipocyte differentiation and toward osteoblast differentiation under healthy and osteoporotic conditions. These findings pave the way for further translational studies to investigate the therapeutic possibilities of JPE in both prevention and treatment of osteoporosis.
Databáze: MEDLINE
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