Determination of vitamin D3 in daily oily supplements by a two-dimensional supercritical fluid chromatography-liquid chromatography-mass spectrometry system.

Autor: Feng L; School of Chemical & Environmental Engineering, China University of Mining and Technology (Beijing), Beijing 100083, China., Wu L; School of Chemical & Environmental Engineering, China University of Mining and Technology (Beijing), Beijing 100083, China., Guo Y; China Innovation Center, Shimadzu (China) Co., Beijing Branch, Beijing 100020, China. Electronic address: spkgyl@shimadzu.com.cn., Hamada N; China Innovation Center, Shimadzu (China) Co., Beijing Branch, Beijing 100020, China., Hashi Y; China Innovation Center, Shimadzu (China) Co., Beijing Branch, Beijing 100020, China., Li X; China Innovation Center, Shimadzu (China) Co., Beijing Branch, Beijing 100020, China., Cao L; China Innovation Center, Shimadzu (China) Co., Beijing Branch, Beijing 100020, China.
Jazyk: angličtina
Zdroj: Journal of chromatography. A [J Chromatogr A] 2020 Oct 11; Vol. 1629, pp. 461510. Date of Electronic Publication: 2020 Aug 25.
DOI: 10.1016/j.chroma.2020.461510
Abstrakt: A two-dimensional system composed of supercritical fluid chromatography (SFC) and reverse phase liquid chromatography (RPLC) coupled a tandem mass spectrometry (MS) was developed for the quantitative analysis of vitamin D in daily oily supplements. Two six-port switching valves are configured, allowing four different valve positions. When the valve positions were fixed at Position A, this system worked at SFC-MS mode. When the valve positions switched between Position B and C, this system worked at a SFC-LC-MS switching mode. Vitamin D3 in two kinds of oily drops, Baby Ddrops and Vitamin AD drops, was determined at both SFC-MS and SFC-LC-MS switching modes by using the same system. The linearity, repeatability and recovery were investigated using the internal and external standard methods for the two modes. The results obtained from the internal standard method are better than those of the external standard method at either mode. The coefficient of determination (r 2 ) for the internal standard method is more than 0.999, with a linear range of 20-1000 μg/L. Both Baby Ddrops and Vitamin AD drops were analyzed with good repeatability (<3.21%) and recovery (94.1%-116.1%) using internal standard method. When calculated by the external standard method, as compared to SFC-MS mode, SFC-LC-MS switching mode has better linearity (r 2 >0.999), repeatability (Baby Ddrops: 4.45%, Vitamin AD drops: 1.12%) and recovery (86.3%-107.1%). The results indicate that the two-dimensional SFC-MS/SFC-LC-MS system is useful for determination of vitamin D in oily drops. If it works at SFC-MS mode, the internal standard is required. When SFC-LC-MS switching mode is used, external standard method can also obtain the accurate results with good precision.
Competing Interests: Declaration of Competing Interest The authors declare that this article content has no conflicts of interest.
(Copyright © 2020. Published by Elsevier B.V.)
Databáze: MEDLINE
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