α-Amylase and α-glucosidase inhibitors from Zanthoxylum chalybeum Engl. root bark.

Autor: Ochieng CO; Department of Chemistry, Maseno University, Maseno, Kenya. Electronic address: otieno.charles9@gmail.com., Nyongesa DW; Department of Chemistry, Maseno University, Maseno, Kenya., Yamo KO; Department of Chemistry, Maseno University, Maseno, Kenya., Onyango JO; School of Chemical Sciences and Technology, Technical University of Kenya, Nairobi, Kenya., Langat MK; Jodrell Laboratory, Natural Capital and Plant Health, Royal Botanic Gardens, Kew, Richmond TW9 3DS, UK., Manguro LAO; Department of Chemistry, Maseno University, Maseno, Kenya.
Jazyk: angličtina
Zdroj: Fitoterapia [Fitoterapia] 2020 Oct; Vol. 146, pp. 104719. Date of Electronic Publication: 2020 Sep 02.
DOI: 10.1016/j.fitote.2020.104719
Abstrakt: A systematic analysis of the root bark of Zanthoxylum chalybeum was conducted to establish the antidiabetic potential of isolated compounds based on its ethnomedicinal use to manage diabetes. Chromatographic separation of alkaloid extracts led to isolation of three undescribed amides, chaylbemide A (1), chalybemide B (2) and chalybemide C (3) alongside the known fagaramide (4); four known benzophenanthridine alkaloids skimmianine (5), norchelerythrine (6), 6-acetonyldihydrochelerythrine (7) and 6-hydroxy-N-methyl decarine (8). The alkaloid free extracts yielded three known lignans, ailanthoidol (9), 2,3-epoxy-6,7-methylenedioxy coniferyl alcohol (10), sesamine (11), together with five known triterpenoids, lupeol (12), lupanone (13), 3α,20-dihydroxy-28-lupanoic acid (14), 20-hydroxy-3-oxo-28-lupanoic acid (15) and 3α,20,28-trihydroxylupane (16). The structures of the compounds were established based on 1D and 2D NMR spectroscopic and mass spectrometric experiments. Compounds 1-8 displayed inhibitory activities against both α-amylase and α-glycosidase in the range of IC 50  = 43.22-49.36 μM which showed no significant (P > 0.05) difference to the positive control acarbose (IC 50  = 42.67; 44.88 μM). The results confirmed anti-hyperglycemic potential of alkaloids from Z. chalybeum which lends credence to its use towards management of diabetes susceptibilities.
Competing Interests: Declaration of Competing Interest There are no conflicts of interest among the authors.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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