Saliva is a reliable, non-invasive specimen for SARS-CoV-2 detection.

Autor:	Vaz SN; Universidade Federal da Bahia (UFBA), Hospital Universitário Professor Edgard Santos (HUPES), Laboratório de Pesquisa em Infectologia (LAPI), Salvador, BA, Brazil. Electronic address: saranunes02@hotmail.com., Santana DS; Universidade Federal da Bahia (UFBA), Hospital Universitário Professor Edgard Santos (HUPES), Laboratório de Pesquisa em Infectologia (LAPI), Salvador, BA, Brazil. Electronic address: dnssan@hotmail.com., Netto EM; Universidade Federal da Bahia (UFBA), Hospital Universitário Professor Edgard Santos (HUPES), Laboratório de Pesquisa em Infectologia (LAPI), Salvador, BA, Brazil. Electronic address: nettoeduardom@hotmail.com., Pedroso C; Universidade Federal da Bahia (UFBA), Hospital Universitário Professor Edgard Santos (HUPES), Laboratório de Pesquisa em Infectologia (LAPI), Salvador, BA, Brazil. Electronic address: cpedrosoj@gmail.com., Wang WK; University of Hawaii at Mānoa (UH Manoa), Department of Tropical Medicine, Medical Microbiology and Pharmacology, Hawaii, USA. Electronic address: wangwk@hawaii.edu., Santos FDA; Universidade Federal da Bahia (UFBA), Hospital Universitário Professor Edgard Santos (HUPES), Laboratório de Pesquisa em Infectologia (LAPI), Salvador, BA, Brazil. Electronic address: fdeminco@gmail.com., Brites C; Universidade Federal da Bahia (UFBA), Hospital Universitário Professor Edgard Santos (HUPES), Laboratório de Pesquisa em Infectologia (LAPI), Salvador, BA, Brazil. Electronic address: crbrites@gmail.com.
Jazyk:	angličtina
Zdroj:	The Brazilian journal of infectious diseases : an official publication of the Brazilian Society of Infectious Diseases [Braz J Infect Dis] 2020 Sep - Oct; Vol. 24 (5), pp. 422-427. Date of Electronic Publication: 2020 Aug 31.
DOI:	10.1016/j.bjid.2020.08.001
Abstrakt:	Background: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of Coronavirus Disease 2019 (COVID-19). Although Real Time Reverse Transcription Polymerase Chain Reaction (qRT-PCR) of respiratory specimens is the gold standard test for detection of SARS-CoV-2 infection, collecting nasopharyngeal swabs causes discomfort to patients and may represent considerable risk for healthcare workers. The use of saliva as a diagnostic sample has several advantages. Objectives: The aim of this study was to validate the use of saliva as a biological sample for diagnosis of COVID-19. Methods: This study was conducted at Infectious Diseases Research Laboratory (LAPI), in Salvador, Brazil. Participants presenting with signs/symptoms suggesting SARS-CoV-2 infection underwent a nasopharyngeal swab (NPS) and/or oropharyngeal swab (OPS), and saliva collection. Saliva samples were diluted in PBS, followed by RNA isolation and RT-Real Time PCR for SARS-CoV-2. Results of conventional vs saliva samples testing were compared. Statistical analyses were performed using Statistical Package for the Social Sciences software (SPSS) version 18.0. Results: One hundred fifty-five participants were recruited and samples pairs of NPS/OPS and saliva were collected. The sensitivity and specificity of RT-PCR using saliva samples were 94.4% (95% CI 86.4-97.8) and 97.62% (95% CI 91.7-99.3), respectively. There was an overall high agreement (96.1%) between the two tests. Conclusions: Use of self-collected saliva samples is an easy, convenient, and low-cost alternative to conventional NP swab-based molecular tests. These results may allow a broader use of molecular tests for management of COVID19 pandemic, especially in resources-limited settings. (Copyright © 2020 Sociedade Brasileira de Infectologia. Published by Elsevier España, S.L.U. All rights reserved.)
Databáze:	MEDLINE
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