Biomarkers in bivalve mollusks and amphipods for assessment of effects linked to cyanobacteria and elodea: Mesocosm study.

Autor: Berezina NA; Zoological Institute, Russian Academy of Sciences (RAS), Universitetskaya Embankment 1, St. Petersburg, 199034, Russia. Electronic address: nadezhda.berezina@zin.ru., Verbitsky VB; Papanin Institute for Biology of Inland Waters, RAS, Borok, 152742, Russia., Sharov AN; Papanin Institute for Biology of Inland Waters, RAS, Borok, 152742, Russia; Saint Petersburg Research Center for Ecological Safety, RAS, Korpusnaya Street 18, St. Petersburg, 197110, Russia., Chernova EN; Saint Petersburg Research Center for Ecological Safety, RAS, Korpusnaya Street 18, St. Petersburg, 197110, Russia., Meteleva NY; Papanin Institute for Biology of Inland Waters, RAS, Borok, 152742, Russia., Malysheva OA; Papanin Institute for Biology of Inland Waters, RAS, Borok, 152742, Russia.
Jazyk: angličtina
Zdroj: Ecotoxicology and environmental safety [Ecotoxicol Environ Saf] 2020 Oct 15; Vol. 203, pp. 110994. Date of Electronic Publication: 2020 Jul 18.
DOI: 10.1016/j.ecoenv.2020.110994
Abstrakt: The effects of cyanobacteria (Aphanizomenon flos-aquae (90%), Microcystis aeruginosa) and dense Elodea canadensis beds on the health endpoints of the amphipod Gmelinoides fasciatus and bivalve mollusc Unio pictorum were examined in mesocosms with simulated summer conditions (July-August 2018) in the environment of the Rybinsk Reservoir (Volga River Basin, Russia). Four treatments were conducted, including one control and three treatments with influencing factors, cyanobacteria and dense elodea beds (separately and combined). After 20 days of exposure, we evaluated the frequency of malformed and dead embryos in amphipods, heart rate (HR) and its recovery (HRR) after stress tests in molluscs as well as heat tolerance (critical thermal maximum or CTMax) in both amphipods and molluscs. The significant effect, such as elevated number of malformed embryos, was recorded after exposure with cyanobacteria (separately and combined with elodea) and presence of microcystins (MC) in water (0.17 μg/l, 40% of the most toxic MC-LR contribution). This study provided evidence that an elevated number (>5% of the total number per female) of malformed embryos in amphipods showed noticeable toxicity effects in the presence of cyanobacteria. The decreased oxygen under the influence of dense elodea beds led to a decrease in HR (and an increase in HRR) in molluscs. The notable effects on all studied biomarkers, embryo malformation frequency and heat tolerance in the amphipod G. fasciatus, as well as the heat tolerance and heart rate in the mollusc U. pictorum, were found when both factors (elodea and cyanobacteria) were combined. The applied endpoints could be further developed for environmental monitoring, but the obtained results support the importance of the combined use of several biomarkers and species, especially in the case of multi-factor environmental stress.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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