n-Butanol production by Saccharomyces cerevisiae from protein-rich agro-industrial by-products.

Autor: Santos BAS; Bioprocess and Metabolic Engineering Laboratory, Department of Food Engineering, School of Food Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil., Azambuja SPH; Bioprocess and Metabolic Engineering Laboratory, Department of Food Engineering, School of Food Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil., Ávila PF; Bioprocess and Metabolic Engineering Laboratory, Department of Food Engineering, School of Food Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil., Pacheco MTB; Applied Chemistry and Nutrition Center, Institute of Food Technology (ITAL), Campinas, SP, Brazil., Goldbeck R; Bioprocess and Metabolic Engineering Laboratory, Department of Food Engineering, School of Food Engineering, University of Campinas (UNICAMP), Campinas, SP, Brazil. goldbeck@unicamp.br.
Jazyk: angličtina
Zdroj: Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] [Braz J Microbiol] 2020 Dec; Vol. 51 (4), pp. 1655-1664. Date of Electronic Publication: 2020 Sep 04.
DOI: 10.1007/s42770-020-00370-6
Abstrakt: n-Butanol is a renewable resource with a wide range of applications. Its physicochemical properties make it a potential substitute for gasoline. Saccharomyces cerevisiae can produce n-butanol via amino acid catabolic pathways, but the use of pure amino acids is economically unfeasible for large-scale production. The aim of this study was to optimize the production of n-butanol by S. cerevisiae from protein-rich agro-industrial by-products (sunflower and poultry offal meals). By-products were characterized according to their total protein and free amino acid contents and subjected to enzymatic hydrolysis. Protein hydrolysates were used as nitrogen sources for the production of n-butanol by S. cerevisiae, but only poultry offal meal hydrolysate (POMH) afforded detectable levels of n-butanol. Under optimized conditions (carbon/nitrogen ratio of 2 and working volume of 60%), 59.94 mg/L of n-butanol was produced using POMH and glucose as substrates. The low-cost agro-industrial by-product showed great potential to be used in the production of n-butanol by S. cerevisiae. Other protein-rich residues may also find application in biofuel production by yeasts.
Databáze: MEDLINE
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