Simultaneous extraction of several targets by using non-toxic dual template molecularly imprinted polymers in vivo and in vitro.

Autor: Zhang JW; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China., He JY; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China., Wang CZ; Tang Center of Herbal Medicine and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL, 60637, USA., Yang FQ; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China., Zhou LD; Basic Medical College, Chongqing Medical University, Chongqing, 400016, China. Electronic address: 102501@cqmu.edu.cn., Zhang QH; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China; Tang Center of Herbal Medicine and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL, 60637, USA. Electronic address: qhzhang@cqu.edu.cn., Xia ZN; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, China., Yuan CS; Tang Center of Herbal Medicine and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL, 60637, USA.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2020 Nov 01; Vol. 219, pp. 121283. Date of Electronic Publication: 2020 Jun 17.
DOI: 10.1016/j.talanta.2020.121283
Abstrakt: In this report, a non-toxic Dual Template Molecularly Imprinted Polymers (DMIPs) was synthesized with quercetin and schisandrin b as template molecules, using deep-eutectic solvents as functional monomers for the first time. The DMIPs were used to efficiently and simultaneously enrich quercetin and schisandrin b from the mixed crude extracts of penthorum and schisandra. The results indicated that the DMIPs exhibited rapid adsorption kinetics (80 min for adsorption equilibrium) and high selectivity. The largest adsorbing capacities to quercetin and schisandrin b were 23.58 mg/g and 41.64 mg/g, respectively. After presaturation with quercetin and schisandrin b, the nontoxic saturated DMIPs were fed to the mice. Blood samples of the mice were taken and both quercetin and schisandrin b were successfully detected. The pharmacokinetics of quercetin and schisandrin b were similar to reports in the literature where mice were directly fed with botanicals. Our study provides a reliable protocol such that DMIPs can be used to separate and enrich several target molecules simultaneously from complex biological systems. Our findings suggested that the DMIPs have potential application as a drug delivery system of compound herbal formulas.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: