Extraction of activated epimedium glycosides in vivo and in vitro by using bifunctional-monomer chitosan magnetic molecularly imprinted polymers and identification by UPLC-Q-TOF-MS.

Autor: Zhang JW; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, PR China., Tan L; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, PR China., Yuan JB; Key Laboratory of Modern Chinese Medicine Preparations, Jiangxi University of Traditional Chinese Medicine, Jiangxi, 330000, China., Qiao RF; Key Laboratory of Modern Chinese Medicine Preparations, Jiangxi University of Traditional Chinese Medicine, Jiangxi, 330000, China., Wang CZ; Tang Center of Herbal Medicine and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL 60637, USA., Yang FQ; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, PR China., Zhou LD; Basic Medical College, Chongqing Medical University, Chongqing, 400016, China. Electronic address: 102501@cqmu.edu.cn., Zhang QH; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, PR China; Tang Center of Herbal Medicine and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL 60637, USA. Electronic address: qhzhang@cqu.edu.cn., Xia ZN; School of Chemistry and Chemical Engineering, Chongqing University, Chongqing, 400044, PR China., Yuan CS; Tang Center of Herbal Medicine and Department of Anesthesia & Critical Care, University of Chicago, Chicago, IL 60637, USA.
Jazyk: angličtina
Zdroj: Talanta [Talanta] 2020 Nov 01; Vol. 219, pp. 121350. Date of Electronic Publication: 2020 Jul 09.
DOI: 10.1016/j.talanta.2020.121350
Abstrakt: In this work, efficient, sensitive bifunctional-monomer chitosan magnetic molecularly imprinted polymers (BCMMIPs) were fabricated and successfully applied to concentrate the metabolites of Epimedium flavonoids in rat testis and bone that were later analyzed using UPLC-Q-TOF-MS. Using chitosan and methacrylic acid as co-functional monomers, BCMMIPs exhibited a large adsorption capacity (7.60 mg/g), fast kinetics (60 min), and good selectivity. Chitosan is bio-compatible and non-toxic, and methacrylic acid provides multiple hydrogen bond donors. The BCMMIPs were injected into rat testis to specifically enrich the total flavonoid metabolites in vivo and were used to extract metabolites from bone in vitro. The results showed that the BCMMIPs coupled with UPLC-Q-TOF-MS successfully identified 28 compounds from testis and 18 compounds from bone, including 19 new compounds. This study provided a reliable protocol for the concentration of metabolites from complex biological samples, and several new metabolites of Epimedium flavonoids were found in vivo and in vitro.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: