Alterations in the gut-associated microbiota of juvenile Caribbean spiny lobsters Panulirus argus (Latreille, 1804) infected with PaV1.

Autor: Zamora-Briseño JA; Departamento de Recursos del Mar, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional-unidad Mérida, Km. 6 Antigua Carretera a Progreso, CORDEMEX, Mérida, Yucatán CP. 97310, Mexico., Cerqueda-García D; Departamento de Recursos del Mar, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional-unidad Mérida, Km. 6 Antigua Carretera a Progreso, CORDEMEX, Mérida, Yucatán CP. 97310, Mexico., Hernández-Velázquez IM; Departamento de Recursos del Mar, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional-unidad Mérida, Km. 6 Antigua Carretera a Progreso, CORDEMEX, Mérida, Yucatán CP. 97310, Mexico., Rivera-Bustamante R; Dirección, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional-unidad Mérida, Km. 6 Antigua Carretera a Progreso, CORDEMEX, Mérida, Yucatán CP. 97310, Mexico., Huchín-Mian JP; Departamento de Biología, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta S/N, Guanajuato 36050, Mexico., Briones-Fourzán P; Universidad Nacional Autónoma de México, Instituto de Ciencias del Mar y Limnología, Unidad Académica de Sistemas Arrecifales, Puerto Morelos, Quintana Roo 77580, Mexico., Lozano-Álvarez E; Universidad Nacional Autónoma de México, Instituto de Ciencias del Mar y Limnología, Unidad Académica de Sistemas Arrecifales, Puerto Morelos, Quintana Roo 77580, Mexico., Rodríguez-Canul R; Departamento de Recursos del Mar, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional-unidad Mérida, Km. 6 Antigua Carretera a Progreso, CORDEMEX, Mérida, Yucatán CP. 97310, Mexico. Electronic address: rossana.rodriguez@cinvestav.mx.
Jazyk: angličtina
Zdroj: Journal of invertebrate pathology [J Invertebr Pathol] 2020 Oct; Vol. 176, pp. 107457. Date of Electronic Publication: 2020 Sep 01.
DOI: 10.1016/j.jip.2020.107457
Abstrakt: The spiny lobster Panulirus argus (Latreille, 1804) is currently affected by an unenveloped, icosahedral, DNA virus termed Panulirus argus virus 1 (PaV1), a virulent and pathogenic virus that produces a long-lasting infection that alters the physiology and behaviour of heavily infected lobsters. Gut-associated microbiota is crucial for lobster homeostasis and well-being, but pathogens could change microbiota composition affecting its function. In PaV1 infection, the changes of gut-associated microbiota are yet to be elucidated. In the present study, we used high-throughput 16S rRNA sequencing technology to compare the bacterial microbiota in intestines of healthy and heavily PaV1-infected male and female juveniles of spiny lobsters P. argus captured in Puerto Morelos Reef lagoon, Quintana Roo, Mexico. We found that basal gut-associated microbiota composition showed a sex-dependent bias, with females being enriched in amplicon sequence variants (ASVs) assigned to Sphingomonas, while males were enriched in the genus Candidatus Hepatoplasma and Aliiroseovarius genera. Moreover, the alpha diversity of microbiota decreased in PaV1-infected lobsters. A significant increase of the genus Candidatus Bacilloplasma was observed in infected lobsters, as well as a significant decrease in Nesterenkonia, Caldalkalibacillus, Pseudomonas, Cetobacterium and Phyllobacterium. We also observed an alteration in the abundances of Vibrio species. Results from this study suggest that PaV1 infection impacts intestinal microbiota composition in Panulirus argus in a sex-dependent manner.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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