| Autor: |
Santos MDV; Department of Biochemistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil., Barros MPS; Department of Microbiology, Instituto Aggeu Magalhães (IAM/FIOCRUZ-PE), Recife, Pernambuco, Brazil., Silveira-Filho VDM; Department of Biology, Federal University of Pernambuco (UPE), Garanhuns, Pernambuco, Brazil., Mendes-Marques CL; Department of Microbiology, Instituto Aggeu Magalhães (IAM/FIOCRUZ-PE), Recife, Pernambuco, Brazil., Lima AVA; Department of Biochemistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil., Silva MVD; Department of Biochemistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil., Leal-Balbino TC; Department of Microbiology, Instituto Aggeu Magalhães (IAM/FIOCRUZ-PE), Recife, Pernambuco, Brazil., Silva MDPCD; Department of Biochemistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil., Paiva PMG; Department of Biochemistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil., Oliveira MBM; Department of Biochemistry, Federal University of Pernambuco, Recife, Pernambuco, Brazil. |
| Abstrakt: |
Life-threatening bacterial infections are a major concern in health care services worldwide. This retrospective study aimed to demonstrate genetic and biochemical diversity in isolates of Acinetobacter baumannii and Pseudomonas aeruginosa from a public hospital in Brazil. A total of 63 isolates collected from different sites of infection and hospital sectors were characterized, and their susceptibility profile to antibiotics was assessed for 18 drugs belonging to 8 antimicrobial categories using the automated BACTEC system. Genetic diversity was assessed using the multiple locus variable number tandem repeat analysis. Among the isolates of A. baumannii , 83% were classified as extensively drug resistant (XDR), and 17 genotypic profiles were identified. About 67% of P. aeruginosa isolates were susceptible to antimicrobials and were distributed into 37 genotypic profiles, revealing genetic heterogeneity. This study has demonstrated the multicolonization of investigated pathogens and the high frequency (95.8%) of multidrug-resistant and XDR, as well as high genetic diversity, among the isolates supporting the continuous need to monitor these species in the hospital environment. |
