Static Magnetic Stimulation Induces Cell-type Specific Alterations in the Viability of SH-SY5Y Neuroblastoma Cell Line.

Autor:	Medeiros HR; PostGraduate Program in Medicine: Medical Sciences, Faculty of Medicine, Universidade do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.; Translational Nucleus: Pain Pharmacology and Neuromodulation, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil.; Embryology and Cell Differentiation Laboratory, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Assumpcao JAF; PostGraduate Program in Medicine: Medical Sciences, Faculty of Medicine, Universidade do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.; Translational Nucleus: Pain Pharmacology and Neuromodulation, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Medeiros LF; Translational Nucleus: Pain Pharmacology and Neuromodulation, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Stapenhorst M; Embryology and Cell Differentiation Laboratory, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Nunes L; Embryology and Cell Differentiation Laboratory, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Henckes NAC; Embryology and Cell Differentiation Laboratory, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Cruz CU; Experimental Laboratory of Hepatology and Gastroenterology, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Fregni F; Laboratory of Neuromodulation, Department of Physical Medicine & Rehabilitation, Spaulding Rehabilitation Hospital & Massachusetts General Hospital Harvard Medical School and Center for Non-invasive Brain Stimulation, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, U.S.A., Sanches PRS; Biomedical Engineering Laboratory, Research and Post Graduate Group, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Oliveira FSO; Embryology and Cell Differentiation Laboratory, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Caumo W; PostGraduate Program in Medicine: Medical Sciences, Faculty of Medicine, Universidade do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.; Translational Nucleus: Pain Pharmacology and Neuromodulation, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Cirne-Lima EO; Embryology and Cell Differentiation Laboratory, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil., Torres ILS; PostGraduate Program in Medicine: Medical Sciences, Faculty of Medicine, Universidade do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil iltorres@hcpa.edu.br.; Translational Nucleus: Pain Pharmacology and Neuromodulation, Experimental Research Center, Hospital de Clínicas de Porto Alegre, Porto Alegre, RS, Brazil.
Jazyk:	angličtina
Zdroj:	Anticancer research [Anticancer Res] 2020 Sep; Vol. 40 (9), pp. 5151-5158.
DOI:	10.21873/anticanres.14518
Abstrakt:	Background/aim: Magnetic stimulation is used in the treatment of a diversity of diseases, but a complete understanding of the underlying mechanisms of action requires further investigation. We examined the effect of static magnetic stimulation (SMS) in different cell lines. Materials and Methods: A culture plate holder with attached NeFeB magnets was developed. Different magnetic field intensities and periods were tested in tumoral and non-tumoral cell lines. To verify the cellular responses to SMS, cell viability, cell death, cell cycle and BDNF expression were evaluated. Results: Exposure of SH-SY5Y cells to SMS for 24 hours led to a decrease in cell viability. Analysis 24 h after stimulation revealed a decrease in apoptotic and double-positive cells, associated with an increase in the number of necrotic cells. Conclusion: The effects of SMS on cell viability are cell type-specific, inducing a decrease in cell viability in SH-SY5Y cells. This suggests that SMS may be a potential tool in the treatment of neuronal tumors. (Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze:	MEDLINE
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