| Autor: |
Meco V; Department of Stress Biology and Plant Pathology, Centro de Edafología y Biología Aplicada del Segura, CSIC, Campus Universitario de Espinardo, 30100 Murcia, Spain., Egea I; Department of Stress Biology and Plant Pathology, Centro de Edafología y Biología Aplicada del Segura, CSIC, Campus Universitario de Espinardo, 30100 Murcia, Spain., Ortíz-Atienza A; Centro de Investigación en Biotecnología Agroalimentaria (BITAL). Universidad de Almería, 04120 Almería, Spain., Drevensek S; Institut de Biologie de l'École Normale Supérieure (IBENS), Paris Sciences et Lettres Research University, F-75005 Paris, France., Esch E; BASF Vegetable Seeds, Napoleonsweg 152, 6083AB Nunhem, The Netherlands., Yuste-Lisbona FJ; Centro de Investigación en Biotecnología Agroalimentaria (BITAL). Universidad de Almería, 04120 Almería, Spain., Barneche F; Institut de Biologie de l'École Normale Supérieure (IBENS), Paris Sciences et Lettres Research University, F-75005 Paris, France., Vriezen W; BASF Vegetable Seeds, Napoleonsweg 152, 6083AB Nunhem, The Netherlands., Bolarin MC; Department of Stress Biology and Plant Pathology, Centro de Edafología y Biología Aplicada del Segura, CSIC, Campus Universitario de Espinardo, 30100 Murcia, Spain., Lozano R; Centro de Investigación en Biotecnología Agroalimentaria (BITAL). Universidad de Almería, 04120 Almería, Spain., Flores FB; Department of Stress Biology and Plant Pathology, Centro de Edafología y Biología Aplicada del Segura, CSIC, Campus Universitario de Espinardo, 30100 Murcia, Spain. |
| Abstrakt: |
Tomato cell wall-associated kinase 1 ( SlWAK1 ) has only been studied in biotic stress response and hence its function in abiotic stress remains unknown. In a screening under salinity of an insertional mutant collection of tomato ( Solanum lycopersicum L.), a mutant exhibiting lower degree of leaf chlorosis than wild type (WT) together with reduced leaf Na + accumulation was selected. Genetic analysis of the mutation revealed that a single T-DNA insertion in the SlWAK1 gene was responsible of the mutant phenotype. Slwak1 null mutant reduced its shoot growth compared with WT, despite its improved Na + homeostasis. SlWAK1 disruption affected osmotic homeostasis, as leaf water content was lower in mutant than in WT under salt stress. In addition, Slwak1 altered the source-sink balance under salinity, by increasing sucrose content in roots. Finally, a significant fruit yield reduction was found in Slwak1 vs. WT under long-term salt stress, mainly due to lower fruit weight. Our results show that disruption of SlWAK1 induces a higher sucrose transport from source leaf to sink root, negatively affecting fruit, the main sink at adult stage. |
