Co-Circulation of Multiple Serotypes of Bluetongue Virus in Zambia.

Autor: Chambaro HM; Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.; Virology Unit, Central Veterinary Research Institute, Lusaka 10101, Zambia.; Ministry of Fisheries and Livestock, Lusaka 10101, Zambia., Sasaki M; Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan., Simulundu E; School of Veterinary Medicine, The University of Zambia, Lusaka 10101, Zambia.; Macha Research Trust, Choma 10101, Zambia., Silwamba I; School of Veterinary Medicine, The University of Zambia, Lusaka 10101, Zambia., Sinkala Y; Ministry of Fisheries and Livestock, Lusaka 10101, Zambia., Gonzalez G; National Virus Reference Laboratory, School of Medicine, Dublin DO4V1W8, Ireland., Squarre D; The University of Edinburgh, Edinburgh EH25 9RG, Scotland, UK.; Department of National Parks and Wildlife, Chilanga 10101, Zambia., Fandamu P; Ministry of Fisheries and Livestock, Lusaka 10101, Zambia., Lubaba CH; Ministry of Fisheries and Livestock, Lusaka 10101, Zambia., Munyeme M; School of Veterinary Medicine, The University of Zambia, Lusaka 10101, Zambia., Maseko A; Ministry of Fisheries and Livestock, Lusaka 10101, Zambia., Chimvwele C; Ministry of Fisheries and Livestock, Lusaka 10101, Zambia., Mataa L; Ministry of Fisheries and Livestock, Lusaka 10101, Zambia., Mooya LE; Virology Unit, Central Veterinary Research Institute, Lusaka 10101, Zambia.; Ministry of Fisheries and Livestock, Lusaka 10101, Zambia., Mukubesa AN; School of Veterinary Medicine, The University of Zambia, Lusaka 10101, Zambia., Harima H; Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan., Samui KL; School of Veterinary Medicine, The University of Zambia, Lusaka 10101, Zambia., Munang'andu HM; Faculty of Veterinary Medicine and Biosciences, Norwegian University of Life Sciences, 0454 Oslo, Norway., Simuunza M; School of Veterinary Medicine, The University of Zambia, Lusaka 10101, Zambia., Nalubamba KS; School of Veterinary Medicine, The University of Zambia, Lusaka 10101, Zambia., Qiu Y; Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, University of Zambia, Lusaka 10101, Zambia., Carr MJ; National Virus Reference Laboratory, School of Medicine, Dublin DO4V1W8, Ireland.; International Collaboration Unit, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan., Hall WW; National Virus Reference Laboratory, School of Medicine, Dublin DO4V1W8, Ireland.; International Collaboration Unit, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.; Global Virus Network, Baltimore, MD 21201, USA., Eshita Y; Hokudai Center for Zoonosis Control in Zambia, School of Veterinary Medicine, University of Zambia, Lusaka 10101, Zambia., Sawa H; Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.; International Collaboration Unit, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.; Global Virus Network, Baltimore, MD 21201, USA., Orba Y; Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.; International Collaboration Unit, Research Center for Zoonosis Control, Hokkaido University, Sapporo 001-0020, Japan.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2020 Aug 31; Vol. 12 (9). Date of Electronic Publication: 2020 Aug 31.
DOI: 10.3390/v12090963
Abstrakt: Bluetongue (BT) is an arthropod-borne viral disease of ruminants with serious trade and socio-economic implications. Although the disease has been reported in a number of countries in sub-Saharan Africa, there is currently no information on circulating serotypes and disease distribution in Zambia. Following surveillance for BT in domestic and wild ruminants in Zambia, BT virus (BTV) nucleic acid and antibodies were detected in eight of the 10 provinces of the country. About 40% (87/215) of pooled blood samples from cattle and goats were positive for BTV nucleic acid, while one hartebeest pool (1/43) was positive among wildlife samples. Sequence analysis of segment 2 revealed presence of serotypes 3, 5, 7, 12 and 15, with five nucleotypes (B, E, F, G and J) being identified. Segment 10 phylogeny showed Zambian BTV sequences clustering with Western topotype strains from South Africa, intimating likely transboundary spread of BTV in Southern Africa. Interestingly, two Zambian viruses and one isolate from Israel formed a novel clade, which we designated as Western topotype 4. The high seroprevalence (96.2%) in cattle from Lusaka and Central provinces and co-circulation of multiple serotypes showed that BT is widespread, underscoring the need for prevention and control strategies.
Databáze: MEDLINE
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