Inhibition of dengue virus serotype 2 in Vero cells with [Cu(2,4,5-triphenyl-1H-imidazole) 2(H2O)2].Cl2.

Autor: Sucipto TH; Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga, Jl. Mulyorejo, Surabaya, East Java., Martak F; Department of Chemistry, Faculty of Science, Institut Teknologi Sepuluh Nopember, Jl. Raya ITS, Surabaya, East Java, Indonesia.
Jazyk: angličtina
Zdroj: Infectious disease reports [Infect Dis Rep] 2020 Jul 07; Vol. 12 (Suppl 1), pp. 8744. Date of Electronic Publication: 2020 Jul 07 (Print Publication: 2020).
DOI: 10.4081/idr.2020.8744
Abstrakt: Dengue fever and dengue hemorrhagic fever are transmitted to humans by the Aedes aegypti and Aedes albopictus mosquitoes, with an observed 30-fold increase in global incidence the last 50 years. Despite the tremendous efforts invested anti-dengue virus research, no clinically approved vaccine or antiviral chemotherapeutics are available for humans, and disease treatment is limited to supportive care. Over the years there has been a continuous interest in the chemistry of metal complexes with biological activity, including platinum complexes with antitumor activity and silver complexes with antimicrobial action. Aim of the project was to investigate [Cu(2,4,5-triphenyl-1 H imidazole) 2 (H 2 O) 2 ].Cl 2 as antiviral compound that was further tested for inhibitory effect on the replication of dengue virus type 2 (DENV-2) in Vero cell. DENV-2 were infected in Vero cells and replication of virus was measured by Viral ToxGlo with selectivity index value (SI) and determined as the ratio of cytotoxic concentration 50 (CC 50 ) to inhibitory concentration 50 (IC 50 ) for compound. The standard curve between concentration of compound (μg/mL) and %viability of cells was analyzed by logarithmic correlation regression with regression equation. For infection rates, t-test was used to examine the statistical significances among the concentrations of compound. P<0.05 was considered to be significant. The maximum inhibitory concentration (IC 50 ) of [Cu(2,4,5- triphenyl-1 H -imidazole) 2 (H 2 O) 2 ].Cl 2 against DENV-2 was 98.62 μg/mL. The cytotoxic concentration (CC 50 ) of compound against Vero cells was 300.36μg/mL. The SI values for [Cu(2,4,5-triphenyl-1 H -imidazole) 2 (H 2 O) 2 ].Cl 2 1.86.Based on selectivity index values, [Cu(2,4,5-triphenyl-1 H -imidazole) 2 (H 2 O) 2 ].Cl 2 can inhibit the growth of DENV- 2 and has low toxicity values for Vero cells.
Competing Interests: Conflict of interest: the authors declare no potential conflict of interest.
(©Copyright: the Author(s).)
Databáze: MEDLINE
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