Expression of Ascorbate Peroxidase Derived from Cyanidioschyzon merolae in Mammalian Cells.

Autor: Hitomi S; Division of Physiology, Kyushu Dental University, Fukuoka, Japan hitomi.suzuro@nihon-u.ac.jp., Kokabu S; Division of Molecular Signaling and Biochemistry, Kyushu Dental University, Kitakyushu, Fukuoka, Japan., Matsumoto KI; Quantitative RedOx Sensing Group, Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, Quantum Medical Science Directorate, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan., Shoji Y; Quantitative RedOx Sensing Group, Department of Basic Medical Sciences for Radiation Damages, National Institute of Radiological Sciences, Quantum Medical Science Directorate, National Institutes for Quantum and Radiological Science and Technology, Chiba, Japan., Ujihara I; Division of Physiology, Kyushu Dental University, Fukuoka, Japan., Ono K; Division of Physiology, Kyushu Dental University, Fukuoka, Japan.
Jazyk: angličtina
Zdroj: In vivo (Athens, Greece) [In Vivo] 2020 Sep-Oct; Vol. 34 (5), pp. 2437-2441.
DOI: 10.21873/invivo.12058
Abstrakt: Background/aim: Ascorbate peroxidase (APX) derived from Cyanidioschyzon merolae, a primitive red alga living in high temperature and acidic environments, has greater anti-oxidative capacity than similar peroxidases occurring in other plants. In the present study, we examined whether expression of Cyanidioschyzon merolae-derived APX (cAPX) in mammalian cells increases cellular anti-oxidative capacity.
Materials and Methods: The cAPX gene was introduced into the mouse fibroblast-like cell line C3H10T1/2. Production of reactive oxygen species (ROS) and/or cell viability was assessed after heat, H 2 O 2 and acid stimulation.
Results: Heat and H 2 O 2 stimulation resulted in ROS production. cAPX-expressing cells were more tolerant to oxidative stress induced by heat, H 2 O 2 and acid stimulations than control cells lacking cAPX.
Conclusion: Introduction of cAPX increases the anti-oxidative capacity in mammalian cells.
(Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
Databáze: MEDLINE
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