Cigarette Smoke Exposure Promotes Virulence of Pseudomonas aeruginosa and Induces Resistance to Neutrophil Killing.

Autor:	Chien J; Pulmonary and Critical Care Section, VA San Diego Healthcare System, La Jolla, California, USA.; Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, University of California San Diego, La Jolla, California, USA., Hwang JH; Pulmonary and Critical Care Section, VA San Diego Healthcare System, La Jolla, California, USA.; Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, University of California San Diego, La Jolla, California, USA., Nilaad S; Pulmonary and Critical Care Section, VA San Diego Healthcare System, La Jolla, California, USA.; Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, University of California San Diego, La Jolla, California, USA., Masso-Silva JA; Pulmonary and Critical Care Section, VA San Diego Healthcare System, La Jolla, California, USA.; Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, University of California San Diego, La Jolla, California, USA., Jeong Ahn S; Pulmonary and Critical Care Section, VA San Diego Healthcare System, La Jolla, California, USA.; Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, University of California San Diego, La Jolla, California, USA., McEachern EK; Pulmonary and Critical Care Section, VA San Diego Healthcare System, La Jolla, California, USA.; Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, University of California San Diego, La Jolla, California, USA., Moshensky A; Pulmonary and Critical Care Section, VA San Diego Healthcare System, La Jolla, California, USA.; Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, University of California San Diego, La Jolla, California, USA., Byun MK; Pulmonary and Critical Care Section, VA San Diego Healthcare System, La Jolla, California, USA.; Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, University of California San Diego, La Jolla, California, USA.; Division of Pulmonology, Department of Internal Medicine, Gangnam Severance Hospital, Yonsei University College of Medicine, Seoul, South Korea., Crotty Alexander LE; Pulmonary and Critical Care Section, VA San Diego Healthcare System, La Jolla, California, USA lca@ucsd.edu.; Division of Pulmonary, Critical Care and Sleep Medicine, Department of Medicine, University of California San Diego, La Jolla, California, USA.
Jazyk:	angličtina
Zdroj:	Infection and immunity [Infect Immun] 2020 Oct 19; Vol. 88 (11). Date of Electronic Publication: 2020 Oct 19 (Print Publication: 2020).
DOI:	10.1128/IAI.00527-20
Abstrakt:	It is widely known that cigarette smoke damages host defenses and increases susceptibility to bacterial infections. Pseudomonas aeruginosa , a Gram-negative bacterium that commonly colonizes the airways of smokers and patients with chronic lung disease, can cause pneumonia and sepsis and can trigger exacerbations of lung diseases. Pseudomonas aeruginosa colonizing airways is consistently exposed to inhaled cigarette smoke. Here, we investigated whether cigarette smoke alters the ability of this clinically significant microbe to bypass host defenses and cause invasive disease. We found that cigarette smoke extract (CSE) exposure enhances resistance to human neutrophil killing, but this increase in pathogenicity was not due to resistance to neutrophil extracellular traps. Instead, Pseudomonas aeruginosa exposed to CSE (CSE-PSA) had increased resistance to oxidative stress, which correlated with increased expression of tpx , a gene essential for defense against oxidative stress. In addition, exposure to CSE induced enhanced biofilm formation and resistance to the antibiotic levofloxacin. Finally, CSE-PSA had increased virulence in a model of pneumonia, with 0% of mice infected with CSE-PSA alive at day 6, while 28% of controls survived. Altogether, these data show that cigarette smoke alters the phenotype of P. aeruginosa , increasing virulence and making it less susceptible to killing by neutrophils and more capable of causing invasive disease. These findings provide further explanation of the refractory nature of respiratory illnesses in smokers and highlight cigarette smoking as a potential driver of virulence in this important airway pathogen. (Copyright © 2020 American Society for Microbiology.)
Databáze:	MEDLINE
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