Production of commercially important enzymes from Bacillus licheniformis KIBGE-IB3 using date fruit wastes as substrate.

Autor: Aslam F; Department of Natural and Basic Sciences, University of Turbat, Turbat, 92600, Pakistan., Ansari A; Dr. A. Q. Khan Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan., Aman A; Dr. A. Q. Khan Institute of Biotechnology and Genetic Engineering (KIBGE), University of Karachi, Karachi, Pakistan., Baloch G; Department of Natural and Basic Sciences, University of Turbat, Turbat, 92600, Pakistan., Nisar G; Department of Natural and Basic Sciences, University of Turbat, Turbat, 92600, Pakistan., Baloch AH; Faculty of Agriculture, Lasbela University of Agriculture, Water and Marine Sciences (LAWMS), Uthal, Pakistan., Rehman HU; Department of Natural and Basic Sciences, University of Turbat, Turbat, 92600, Pakistan. haneef.baloch@uot.edu.pk.
Jazyk: angličtina
Zdroj: Journal, genetic engineering & biotechnology [J Genet Eng Biotechnol] 2020 Aug 31; Vol. 18 (1), pp. 46. Date of Electronic Publication: 2020 Aug 31.
DOI: 10.1186/s43141-020-00060-8
Abstrakt: Background: Pakistan is one of the top five date fruit-producing countries and produced more than 30% wastes in picking, packing, storage, and commercialization stages. The date fruit wastes are usually considered inedible for humans and only used for livestock feed. In current research, Bacillus licheniformis KIBGE-IB3 was screened for pectinase, xylanase, cellulase, and amylase production using date fruit wastes as substrate through solid state fermentation.
Results: The B. licheniformis KIBGE-IB3 produced higher concentration of pectinase using date fruit wastes as substrate as compared to amylase, cellulase, and xylanase. B. licheniformis KIBGE-IB3 produced maximum pectinase using 5.0 g/dl date fruit wastes and 0.5 g/dl yeast extract. B. licheniformis KIBGE-IB3 required pH 7.0, 37 °C incubation temperature, and 72 h incubation period for maximum production of pectinase.
Conclusion: It has been concluded that date fruit waste is a good source of biomass and can be utilized for the commercial production of pectinase.
Databáze: MEDLINE
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