| Autor: |
Sandin JN; Department of Mechanical Engineering, University of Kentucky., Aryal SP; Department of Chemistry, University of Kentucky., Wilkop T; Department of Physiology, University of Kentucky; UK Light Microscopy Core, University of Kentucky., Richards CI; Department of Chemistry, University of Kentucky; UK Light Microscopy Core, University of Kentucky., Grady ME; Department of Mechanical Engineering, University of Kentucky; m.grady@uky.edu. |
| Jazyk: |
angličtina |
| Zdroj: |
Journal of visualized experiments : JoVE [J Vis Exp] 2020 Aug 11 (162). Date of Electronic Publication: 2020 Aug 11. |
| DOI: |
10.3791/61433 |
| Abstrakt: |
Techniques available for micro- and nano-scale mechanical characterization have exploded in the last few decades. From further development of the scanning and transmission electron microscope, to the invention of atomic force microscopy, and advances in fluorescent imaging, there have been substantial gains in technologies that enable the study of small materials. Conpokal is a portmanteau that combines confocal microscopy with atomic force microscopy (AFM), where a probe "pokes" the surface. Although each technique is extremely effective for the qualitative and/or quantitative image collection on their own, Conpokal provides the capability to test with blended fluorescence imaging and mechanical characterization. Designed for near simultaneous confocal imaging and atomic force probing, Conpokal facilitates experimentation on live microbiological samples. The added insight from paired instrumentation provides co-localization of measured mechanical properties (e.g., elastic modulus, adhesion, surface roughness) by AFM with subcellular components or activity observable through confocal microscopy. This work provides a step by step protocol for the operation of laser scanning confocal and atomic force microscopy, simultaneously, to achieve same cell, same region, confocal imaging, and mechanical characterization. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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