Spin∞: an updated miniaturized spinning bioreactor design for the generation of human cerebral organoids from pluripotent stem cells.

Autor:	Romero-Morales AI; Department of Cell & Developmental Biology, Vanderbilt University, Nashville, TN, USA., O'Grady BJ; Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA.; Interdisciplinary Materials Science Program, Vanderbilt University, Nashville, TN, USA.; Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, USA., Balotin KM; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA., Bellan LM; Department of Mechanical Engineering, Vanderbilt University, Nashville, TN, USA.; Interdisciplinary Materials Science Program, Vanderbilt University, Nashville, TN, USA.; Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, USA., Lippmann ES; Department of Chemical and Biomolecular Engineering, Vanderbilt University, Nashville, TN, USA.; Department of Biomedical Engineering, Vanderbilt University, Nashville, TN, USA.; Vanderbilt Center for Stem Cell Biology, Vanderbilt University, Nashville, TN, USA.; Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN, USA., Gama V; Department of Cell & Developmental Biology, Vanderbilt University, Nashville, TN, USA.; Vanderbilt Center for Stem Cell Biology, Vanderbilt University, Nashville, TN, USA.; Vanderbilt Brain Institute, Vanderbilt University, Nashville, TN, USA.; Vanderbilt Ingram Cancer Center, Vanderbilt University, Nashville, TN, USA.
Jazyk:	angličtina
Zdroj:	HardwareX [HardwareX] 2019 Oct; Vol. 6. Date of Electronic Publication: 2019 Oct 11.
DOI:	10.1016/j.ohx.2019.e00084
Abstrakt:	Three-dimensional (3D) brain organoids derived from human pluripotent stem cells (hPSCs), including human embryonic stem cells (hESCs) and induced pluripotent stem cells (iPSCs), have become a powerful system to study early development events and to model human disease. Cerebral organoids are generally produced in static culture or in a culture vessel with active mixing, and the two most widely used systems for mixing are a large spinning flask and a miniaturized multi-well spinning bioreactor (also known as Spin Omega (SpinΩ)). The SpinΩ provides a system that is amenable to drug testing, has increased throughput and reproducibility, and utilizes less culture media. However, technical limitations of this system include poor stability of select components and an elevated risk of contamination due to the inability to sterilize the device preassembled. Here, we report a new design of the miniaturized bioreactor system, which we term Spinfinity (Spin∞) that overcomes these concerns to permit long-term experiments. This updated device is amenable to months-long (over 200 days) experiments without concern of unexpected malfunctions. Competing Interests: Declaration of interest The authors have no conflict of interest to declare.
Databáze:	MEDLINE
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