The effect of temperature and time on the viability of Taenia solium metacestodes in pork.

Autor: Møller KS; Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlægevej 100, 1870 Frederiksberg C, Denmark.. Electronic address: karenm@sund.ku.dk., Ngowi H; Department of Veterinary Medicine and Public Health, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture, P.O. Box 3021, Morogoro, Tanzania., Magnussen P; Centre for Medical Parasitology, Department of Immunology and Microbiology, Faculty of Health and Medical Sciences, University of Copenhagen, Building 7-11-50, Blegdamsvej 3 B, 2200 Copenhagen, N, Denmark.. Electronic address: pma@sund.ku.dk., Magne J; Danish School of Education (DPU), Campus Emdrup, Tuborgvej 164, Building D 115, 2400 Copenhagen, NV, Denmark.. Electronic address: jema@edu.au.dk., Kabululu M; Tanzania Livestock Research Institute (TALIRI) - Uyole, P.O. Box 6191, Mbeya, Tanzania., Johansen MV; Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, Dyrlægevej 100, 1870 Frederiksberg C, Denmark.
Jazyk: angličtina
Zdroj: Veterinary parasitology, regional studies and reports [Vet Parasitol Reg Stud Reports] 2020 Jul; Vol. 21, pp. 100436. Date of Electronic Publication: 2020 Jul 17.
DOI: 10.1016/j.vprsr.2020.100436
Abstrakt: Objective: Taenia solium taeniosis is a growing health problem in large parts of the world including Sub-Saharan Africa. Humans are infected by eating undercooked pork with T. solium metacestodes, which cause taeniosis. The aim of the present study was to assess the effect of increasing temperatures on T. solium metacestode viability in pork.
Methods: Heavily T. solium infected pieces of pork were cooked in a water-bath at five different temperatures (40 °C, 50 °C, 60 °C, 70 °C and 80 °C) for 10-60 min. At each temperature/time point, five 5x5x5 cm pieces of pork fitted with thermometers at the core were placed in the water-bath. Controls were kept at 5 °C throughout exposure. After exposure, approximately 100 intact metacestodes were harvested and after a maximum of 6 h incubation at 37 °C in a culture media consisting of 50% porcine bile and 50% saline, the metacestodes were evaluated for viability.
Results: The metacestodes were fully viable after cooking at a core temperature of 40 °C for up to an hour. The metacestodes were non-viable after cooking for >40 min at a core temperature over 50 °C. All metacestodes were dead after cooking for 30 min at a core temperature of 60 °C; at 70 °C, non-viability was found after 20 min and all metacestodes were dead after 10 min cooking at 80 °C.
Conclusion: Findings showed that pork pieces cooked at >80 °C for >10 min proved safe for human consumption. This means that dishes consisting of pork pieces in sizes not greater than a 5 cm cube which are immersed in continuously boiling water for at least 10 min would be safe to eat. However, pork deep-fried in oil may entail a risk due to generally shorter cooking time. More research on the cooking practises is needed in order to produce safe guidelines for risk-free pork consumption.
Competing Interests: Declarations of interest None.
(Copyright © 2020. Published by Elsevier B.V.)
Databáze: MEDLINE
Externí odkaz: