Immune response related to Pelibuey sheep naturally infected with gastrointestinal nematodes in a tropical region of Mexico.

Autor: Maza-Lopez J; Facultad de Ingenieros en Biotecnología, Universidad Politécnica del Estado de Morelos, Jiutepec, Morelos, Mexico., Pacheco-Armenta MJ; Facultad de Ingenieros en Biotecnología, Universidad Politécnica del Estado de Morelos, Jiutepec, Morelos, Mexico., Reyes-Guerrero DE; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Carr. Fed. Cuernavaca-Cuautla No. 8254, Jiutepec, Morelos C.P. 62550, Mexico., Olmedo-Juárez A; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Carr. Fed. Cuernavaca-Cuautla No. 8254, Jiutepec, Morelos C.P. 62550, Mexico., González-Garduño R; Universidad Autónoma de Chapingo, Unidad Regional Universitaria Sur-Sureste, Km 7.5, Carr. Teapa-Vicente Guerrero, Tabasco, Mexico., Olazarán-Jenkins S; Sitio Experimental Las Margaritas, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias, Hueytamalco, Pue., Mexico., López-Arellano ME; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias. Carr. Fed. Cuernavaca-Cuautla No. 8254, Jiutepec, Morelos C.P. 62550, Mexico. Electronic address: lopez.mariaeugenia@inifap.gob.mx.
Jazyk: angličtina
Zdroj: Veterinary parasitology, regional studies and reports [Vet Parasitol Reg Stud Reports] 2020 Jul; Vol. 21, pp. 100422. Date of Electronic Publication: 2020 Jun 13.
DOI: 10.1016/j.vprsr.2020.100422
Abstrakt: We analysed the immune response involved in sheep naturally infected with gastrointestinal (GI) nematodes. Fifteen Pelibuey lambs were grazed in paddocks contaminated with GI nematodes for 13 weeks. To assess the infection, the number of eggs per gram (epg) and the percentage of packed cell volume (pcv) were evaluated. Blood and abomasal tissue samples were collected at week 8 post-infection to analyse the expression levels of IL-4, IL-5, IL-6, IL-8, IL-13, TGF-β and FCεR1A genes. The nematode Haemonchus contortus was the main species identified. In addition, two groups of lambs were classified based on the x ± SE of epg and pcv values: G-1, with 151 ± 28 and 29 ± 0.33%, respectively, and G-2, with 475 ± 59.5 and 26 ± 0.38%, respectively. For G-1, upregulation of IL-4, IL-8, IL-13, TGF-β and FCεR1A genes from 2.42- to 14.99-fold was observed in blood and abomasal tissue samples (p > .05), and IL-5, IL-8 and TGF-β genes had significant gene expression levels in blood (p < .05). For G-2, moderate gene expression levels, ranging from 1.22- to 3.45-fold, were observed in abomasal tissue (p > .05), and the IL-5 gene presented significant gene expression in blood (p < .05). Strong positively correlated values (r) between pcv and IL-4, IL-8 and TGF-β genes were observed in G-1. In contrast, significant negative correlations between epg and IL-4, IL-5 and FCεR1A genes indicate acute infection for G-2. Our results suggest that IL-4, IL-5, IL-13, TGF-β and FCεR1A genes are important modulators of GI nematode infections of Pelibuey lambs.
Competing Interests: Declaration of Competing Interest The authors declare that they no competing interests.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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