Multivariate probing of antitumor metal-based complexes damage on living cells through Raman imaging.

Autor: Mamián-López MB; Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, 05508-000, SP, Brazil; Federal University of ABC, Av. dos Estados, 5001, 09210-580 Santo André, SP, Brazil. Electronic address: monikml@gmail.com., Bernardi Miguel R; Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, 05508-000, SP, Brazil., Araki K; Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, 05508-000, SP, Brazil., A Temperini ML; Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, 05508-000, SP, Brazil., da Costa Ferreira AM; Departamento de Química Fundamental, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes 748, 05508-000, SP, Brazil.
Jazyk: angličtina
Zdroj: Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy [Spectrochim Acta A Mol Biomol Spectrosc] 2021 Jan 05; Vol. 244, pp. 118838. Date of Electronic Publication: 2020 Aug 17.
DOI: 10.1016/j.saa.2020.118838
Abstrakt: Intracellular modifications caused by two metal-based antitumor compounds were assessed by confocal Raman imaging assisted by multivariate curve resolution method, a very powerful deconvolution tool that can be used to extract the characteristic spectral profile of the individual or "purest" components from an image dataset. The use of this Raman methodology has the advantage of being non-invasive and totally label-free. Four main different intracellular processes were observed under the Raman imaging and multivariate approach combination, and even, significant differences could be identified between the treatments with both metallodrugs. Leakage of the nucleus and nucleolus content into the cytoplasm, along with releasing of cytochrome c were observed for the treatment with the Cu-based complex. At the same time, changes of hydrogen-bonding network were also evidenced, indicating an apoptotic cellular death process, consistent with complementary Total Reflection X-Ray fluorescence (TXRF) and fluorescence experiments attesting mitochondria and DNA as main targets after uptake of the complex by cells. For treatment with the Zn-based complex, changes associated with cytochrome c were not detected, neither a rapid leakage of nucleus content upon 24 h treatment. The hydrogen-bonding network also followed a quite different pattern, suggesting that with this metallodrug, the cellular death follows a different mechanism.
Competing Interests: Declaration of competing interest The authors declare no competing financial interest. Competing financial interests The University of São Paulo and FAPESP have a Brazilian patent based on the antitumor activity of oxindolimine-metal complexes studied in our lab (PI 0600985-9, granted at March 24th, 2020).
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
Externí odkaz: