Development and evaluation of reparative tricalcium silicate-ZrO 2 -Biosilicate composites.

Autor: Queiroz MB; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Sao Paulo, Brazil., Torres FFE; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Sao Paulo, Brazil., Rodrigues EM; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Sao Paulo, Brazil., Viola KS; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Sao Paulo, Brazil., Bosso-Martelo R; Department of Dentistry, School of Dentistry, Bahia Federal University (UFBA), Salvador, Bahia, Brazil., Chavez-Andrade GM; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Sao Paulo, Brazil., Souza MT; CeRTEV-Center for Research, Technology and Education in Vitreous Materials, Vitreous Materials Laboratory (LaMaV), Department of Materials Engineering, Federal University of São Carlos (UFSCar), São Carlos, Sao Paulo, Brazil., Zanotto ED; CeRTEV-Center for Research, Technology and Education in Vitreous Materials, Vitreous Materials Laboratory (LaMaV), Department of Materials Engineering, Federal University of São Carlos (UFSCar), São Carlos, Sao Paulo, Brazil., Guerreiro-Tanomaru JM; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Sao Paulo, Brazil., Tanomaru-Filho M; Department of Restorative Dentistry, School of Dentistry, São Paulo State University (UNESP), Araraquara, Sao Paulo, Brazil.
Jazyk: angličtina
Zdroj: Journal of biomedical materials research. Part B, Applied biomaterials [J Biomed Mater Res B Appl Biomater] 2021 Apr; Vol. 109 (4), pp. 468-476. Date of Electronic Publication: 2020 Aug 28.
DOI: 10.1002/jbm.b.34714
Abstrakt: Biosilicate is a bioactive glass-ceramic used in medical and dental applications. This study evaluated novel reparative materials composed of pure tricalcium silicate (TCS), 30% zirconium oxide (ZrO 2 ) and 10 or 20% biosilicate, in comparison with Biodentine. Setting time was evaluated based on ISO 6876 standard, radiopacity by radiographic analysis, solubility by mass loss, and pH by using a pH meter. Cytotoxicity was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and NR assays. Alkaline phosphatase (ALP) activity and alizarin red were used to evaluate cell bioactivity. Antimicrobial activity was assessed on Enterococcus faecalis by the direct contact test. The data were submitted to analysis of variance (ANOVA)/Tukey; Bonferroni and Kruskal-Wallis, and Dunn tests (α = 0.05). The association of Biosilicate with TCS + ZrO 2 had appropriate setting time, radiopacity, and solubility, alkaline pH, and antimicrobial activity. TCS and Biodentine showed higher ALP activity in 14 days than the control (serum-free medium). All cements produced mineralized nodules. In conclusion, Biosilicate + TCS ZrO 2 decreased the setting time and increased the radiopacity in comparison to TCS. Biosilicate + TCS ZrO2 presented lower solubility and higher radiopacity than Biodentine. In addition, these experimental cements promoted antimicrobial activity and mineralization nodules formation, suggesting their potential for clinical use.
(© 2020 Wiley Periodicals LLC.)
Databáze: MEDLINE
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