Selective flexible packaging pathways of the segmented genome of influenza A virus.

Autor:	Haralampiev I; Molecular Biophysics, Department for Biology, IRI Life Sciences, Humboldt-Universität zu Berlin, Invalidenstr. 42, 10115, Berlin, Germany.; Crystallography, Max Delbrück Center for Molecular Medicine, Robert-Rössle Str. 10, 13125, Berlin, Germany., Prisner S; Molecular Biophysics, Department for Biology, IRI Life Sciences, Humboldt-Universität zu Berlin, Invalidenstr. 42, 10115, Berlin, Germany., Nitzan M; School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel., Schade M; Molecular Biophysics, Department for Biology, IRI Life Sciences, Humboldt-Universität zu Berlin, Invalidenstr. 42, 10115, Berlin, Germany., Jolmes F; Molecular Biophysics, Department for Biology, IRI Life Sciences, Humboldt-Universität zu Berlin, Invalidenstr. 42, 10115, Berlin, Germany.; PicoQuant, Rudower Chaussee 29, 12489, Berlin, Germany., Schreiber M; Competence Centre Biomedical Data Science, Institute for Applied Informatics e.V. at Leipzig University, Goerdelerring 9, 04109, Leipzig, Germany., Loidolt-Krüger M; Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.; PicoQuant, Rudower Chaussee 29, 12489, Berlin, Germany., Jongen K; Molecular Biophysics, Department for Biology, IRI Life Sciences, Humboldt-Universität zu Berlin, Invalidenstr. 42, 10115, Berlin, Germany., Chamiolo J; Bioorganic Synthesis, Department for Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489, Berlin, Germany., Nilson N; Molecular Biophysics, Department for Biology, IRI Life Sciences, Humboldt-Universität zu Berlin, Invalidenstr. 42, 10115, Berlin, Germany., Winter F; Department of NanoBiophotonics, Max-Planck-Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Göttingen, Germany.; Abberior Instruments GmbH, Hans-Adolf-Krebs-Weg 1, 37077, Göttingen, Germany., Friedman N; School of Computer Science and Engineering, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel.; Institute of Life Sciences, The Hebrew University of Jerusalem, Jerusalem, 91904, Israel., Seitz O; Bioorganic Synthesis, Department for Chemistry, Humboldt-Universität zu Berlin, Brook-Taylor-Straße 2, 12489, Berlin, Germany., Wolff T; Robert Koch Institut, Unit 17, Influenza and Other Respiratory Viruses, Seestraße 10, 13353, Berlin, Germany. WolffT@RKI.de., Herrmann A; Molecular Biophysics, Department for Biology, IRI Life Sciences, Humboldt-Universität zu Berlin, Invalidenstr. 42, 10115, Berlin, Germany. andreas.herrmann@rz.hu-berlin.de.
Jazyk:	angličtina
Zdroj:	Nature communications [Nat Commun] 2020 Aug 28; Vol. 11 (1), pp. 4355. Date of Electronic Publication: 2020 Aug 28.
DOI:	10.1038/s41467-020-18108-1
Abstrakt:	The genome of influenza A viruses (IAV) is encoded in eight distinct viral ribonucleoproteins (vRNPs) that consist of negative sense viral RNA (vRNA) covered by the IAV nucleoprotein. Previous studies strongly support a selective packaging model by which vRNP segments are bundling to an octameric complex, which is integrated into budding virions. However, the pathway(s) generating a complete genome bundle is not known. We here use a multiplexed FISH assay to monitor all eight vRNAs in parallel in human lung epithelial cells. Analysis of 3.9 × 10 5 spots of colocalizing vRNAs provides quantitative insights into segment composition of vRNP complexes and, thus, implications for bundling routes. The complexes rarely contain multiple copies of a specific segment. The data suggest a selective packaging mechanism with limited flexibility by which vRNPs assemble into a complete IAV genome. We surmise that this flexibility forms an essential basis for the development of reassortant viruses with pandemic potential.
Databáze:	MEDLINE
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