| Autor: |
Hasegawa Y; Department of Food Science and Technology, University of California-Davis, Davis, CA 95616, USA., Otoki Y; Department of Food Science and Technology, University of California-Davis, Davis, CA 95616, USA.; Graduate School of Agricultural Science, Tohoku University, Sendai, Miyagi 980-8572, Japan., McClorry S; Department of Nutrition, University of California-Davis, Davis, CA 95616, USA., Coates LC; Department of Food Science and Technology, University of California-Davis, Davis, CA 95616, USA., Lombardi RL; Department of Food Science and Technology, University of California-Davis, Davis, CA 95616, USA., Taha AY; Department of Food Science and Technology, University of California-Davis, Davis, CA 95616, USA., Slupsky CM; Department of Food Science and Technology, University of California-Davis, Davis, CA 95616, USA.; Department of Nutrition, University of California-Davis, Davis, CA 95616, USA. |
| Abstrakt: |
A more comprehensive picture of tissue biology can be obtained through the application and integration of multiple omic technologies. However, the common challenge in working with a precious sample is having a sample too small to separately extract analytes of interest for each experiment. Considering the high heterogeneity that can be present in a single tissue sample, extracting all biomolecules from a single and undivided tissue is preferable because it allows direct comparison of results. Here, we combined a modified Folch extraction method with DNA, RNA, small RNA, and protein extraction using two commercial kits, which allowed us to extract polar metabolites and non-polar oxylipin metabolites, DNA, RNA, small RNA, and protein simultaneously from a small tissue sample. The method was validated in terms of quantity and quality of analytes for downstream analyses. |
