Long term cryopreservation of the amastigote stages of hemoflagellates.

Autor: Yaeger RG; Department of Tropical Medicine, Tulane University School of Public Health and Tropical Medicine, New Orleans, Louisiana 70112.
Jazyk: angličtina
Zdroj: The Journal of protozoology [J Protozool] 1988 Feb; Vol. 35 (1), pp. 114-5.
DOI: 10.1111/j.1550-7408.1988.tb04088.x
Abstrakt: Tissue homogenates containing amastigotes of either Leishmania donovani, L. tropica, or Trypanosoma cruzi were rapidly frozen with 10% glycerol as cryoprotectant. Viability and pathogenicity were maintained for at least 23 years with the Khartoum strain of L. donovani, 22 years with the Malakal strain of L. donovani, and 7 years for L. tropica and T. cruzi. Similar results over a shorter period of time were obtained with a slow-freezing technique.
Databáze: MEDLINE