Systematic Immunophenotyping Reveals Sex-Specific Responses After Painful Injury in Mice.

Autor:	Tawfik VL; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, United States., Huck NA; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, United States., Baca QJ; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, United States., Ganio EA; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, United States., Haight ES; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, United States., Culos A; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, United States., Ghaemi S; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, United States.; Digital Technologies Research Centre, National Research Council Canada, Toronto, ON, Canada., Phongpreecha T; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, United States.; Department of Biomedical Data Sciences, Stanford University, Stanford, CA, United States.; Department of Pathology, Stanford University, Stanford, CA, United States., Angst MS; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, United States., Clark JD; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, United States., Aghaeepour N; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, United States.; Department of Biomedical Data Sciences, Stanford University, Stanford, CA, United States.; Department of Pediatrics, Stanford University, Stanford, CA, United States., Gaudilliere B; Department of Anesthesiology, Perioperative and Pain Medicine, Stanford University, Stanford, CA, United States.; Department of Pediatrics, Stanford University, Stanford, CA, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in immunology [Front Immunol] 2020 Jul 29; Vol. 11, pp. 1652. Date of Electronic Publication: 2020 Jul 29 (Print Publication: 2020).
DOI:	10.3389/fimmu.2020.01652
Abstrakt:	Many diseases display unequal prevalence between sexes. The sex-specific immune response to both injury and persistent pain remains underexplored and would inform treatment paradigms. We utilized high-dimensional mass cytometry to perform a comprehensive analysis of phenotypic and functional immune system differences between male and female mice after orthopedic injury. Multivariate modeling of innate and adaptive immune cell responses after injury using an elastic net algorithm, a regularized regression method, revealed sex-specific divergence at 12 h and 7 days after injury with a stronger immune response to injury in females. At 12 h, females upregulated STAT3 signaling in neutrophils but downregulated STAT1 and STAT6 signals in T regulatory cells, suggesting a lack of engagement of immune suppression pathways by females. Furthermore, at 7 days females upregulated MAPK pathways (p38, ERK, NFkB) in CD4T memory cells, setting up a possible heightened immune memory of painful injury. Taken together, our findings provide the first comprehensive and functional analysis of sex-differences in the immune response to painful injury. (Copyright © 2020 Tawfik, Huck, Baca, Ganio, Haight, Culos, Ghaemi, Phongpreecha, Angst, Clark, Aghaeepour and Gaudilliere.)
Databáze:	MEDLINE
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