Biotransformation of cladribine by a nanostabilized extremophilic biocatalyst.

Autor: Rivero CW; Laboratory of Sustainable Biotechnology (LIBioS), National University of Quilmes, Roque Sáenz Peña 352, Bernal, B1876BXD, Argentina; National Scientific and Technical Research Council (CONICET), Godoy Cruz 2290, CABA, C1425FQB, Argentina., De Benedetti EC; Laboratory of Sustainable Biotechnology (LIBioS), National University of Quilmes, Roque Sáenz Peña 352, Bernal, B1876BXD, Argentina; National Scientific and Technical Research Council (CONICET), Godoy Cruz 2290, CABA, C1425FQB, Argentina., Sambeth J; National Scientific and Technical Research Council (CONICET), Godoy Cruz 2290, CABA, C1425FQB, Argentina; Center for Research and Development in Applied Sciences 'Dr. Jorge J. Ronco', National University of La Plata, La Plata, Argentina., Trelles JA; Laboratory of Sustainable Biotechnology (LIBioS), National University of Quilmes, Roque Sáenz Peña 352, Bernal, B1876BXD, Argentina; National Scientific and Technical Research Council (CONICET), Godoy Cruz 2290, CABA, C1425FQB, Argentina. Electronic address: jtrelles@unq.edu.ar.
Jazyk: angličtina
Zdroj: Journal of biotechnology [J Biotechnol] 2020 Nov 10; Vol. 323, pp. 166-173. Date of Electronic Publication: 2020 Aug 22.
DOI: 10.1016/j.jbiotec.2020.08.012
Abstrakt: Cladribine (2-chloro-2'-deoxy-β-d-adenosine) is a 2'-deoxyadenosine analogue, approved by the FDA for the treatment of hairy cell leukemia and more recently has been proved for therapeutic against many autoimmune diseases as multiple sclerosis. The biosynthesis of this compound using Thermomonospora alba CECT 3324 as biocatalyst is herein reported. This thermophilic microorganism was successfully entrapped in polyacrylamide gel supplemented with nanoclays such as bentonite. The immobilized biocatalyst (T. alba-Ac-Bent 1.00 %), was able to biosynthesize cladribine with a conversion of 89 % in 1 h of reaction and retains its activity for more than 270 reuses without significantly activity loss, showing better operational stability and mechanical properties than the natural matrix. A microscale assay using the developed system, could allow the production of at least 181 mg of cladribine in successive bioprocesses.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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