| Autor: |
Sorho F; Université Félix Houphouët-Boigny, 89247, UFR biosciences, 22 BP 582 Abidjan 22, Abidjan, Côte d'Ivoire, .; Université Félix Houphouët-Boigny, 89247, WASCAL/ CEA CCBAD, Abidjan, Côte d'Ivoire; fsorho@gmail.com., Sereme D; Institut de l'Environnement et de Recherches Agricoles (INERA), Laboratoire de Virologie et de Biotechnologies Végétales, Ouagadougou, Burkina Faso; sereme@gmail.com., Kouamé KD; Université Félix Houphouët-Boigny, 89247, Laboratoire de Physiologie Végétale, UFR Biosciences, Abidjan, Côte d'Ivoire; didykonan@yahoo.fr., Koné N; Université Félix Houphouët-Boigny, 89247, WASCAL/ CEA CCBAD, Abidjan, Côte d'Ivoire; konenahoua06@gmail.com., Yao KJE; Université Félix Houphouët-Boigny, 89247, WASCAL/ CEA CCBAD, Abidjan, Côte d'Ivoire; djojacques87@yahoo.fr., Ouattara MM; Université Félix Houphouët-Boigny, 89247, WASCAL/ CEA CCBAD, Abidjan, Côte d'Ivoire; ouattaramign@gmail.com., Tapsoba WP; Institut de l'Environnement et de Recherches Agricoles (INERA), Laboratoire de Virologie et de Biotechnologies Végétales, Ouagadougou, Kadiogo, Burkina Faso; twendpanga@yahoo.fr., Ouattara B; Institut de l'Environnement et de Recherches Agricoles (INERA), Laboratoire de Virologie et de Biotechnologies Végétales, Ouagadougou, Kadiogo, Burkina Faso; bakaryouatt92@yahoo.fr., Kone D; Université Félix Houphouët-Boigny, 89247, UFR Biosciences, WASCAL/CEA CCBAD, Abidjan, Lagunes, Côte d'Ivoire; daoudakone2013@gmail.com. |
| Jazyk: |
angličtina |
| Zdroj: |
Plant disease [Plant Dis] 2020 Aug 25. Date of Electronic Publication: 2020 Aug 25. |
| DOI: |
10.1094/PDIS-07-19-1398-PDN |
| Abstrakt: |
Sugarcane streak mosaic virus (SCSMV; Poaceavirus; Potiviridae) is the causal agent of streak mosaic disease of sugarcane (Saccharum interspecific hybrids), a major industrial crop that is widely cultivated in tropical and subtropical regions for sugar and ethanol production. was first reported by Hall et al. (1998) from quarantined germplasm material exhibiting mosaic symptoms imported from Pakistan into the USA. Subsequently, the virus was also reported to occur in most of the Asian countries like Bangladesh, India, Indonesia, Iran, Sri Lanka, Thailand, Vietnam and China (Chatenet et al. 2005; Hema et al. 2008, Kasemsin et al. 2016, Putra et al. 2014, Xu et al. 2010, Moradi et al. 2015; Moradi et al. 2018, Zhang et al. 2018). Until now, there is no report of SCSMV outside the Asian continent. From February to October 2018, sugarcane plants exhibiting symptoms such as irregular yellow and green mosaic, interveinal chlorotic specks, and streaks were observed in Bafing (Borotou-Koro), Marahoué (Zuénoula) and Tchologo (Ferkéssédougou) regions of Côte d'Ivoire (Fig. 1a). Varieties under large-scale commercial cultivation such as R570, R579, SP711406, Co997, Co449, M1176/77, M2593/92, M2580/95, and M1400/86 were all symptomatic. A total of 94 sugarcane leaf samples were collected from these regions and, among those, 82 showed disease symptoms and 12 were symptomless. Samples were first tested for the presence of sugarcane mosaic virus (SCMV), which causes mosaic a disease that is already present in Africa. Serological tests with infected sap using a Double Antibody Sandwich (DAS)- Enzyme Linked Immuno Sorbent Assay (ELISA) kit (DSMZ, RT-0166, Braunschweig, Germany) were negative for SCMV and no amplification product was obtained by RT-PCR using primers specific to the coat protein (CP) gene of SCMV (Putra et al. 2003). The 82 symptomatic leaves tested positive by DAS-ELISA with SCSMV antiserum (polyclonal antibodies were graciously provided by Prof. Hema M. of the Sri Venkateswara University, Tirupati, AP, India), whereas the 12 symptomless samples tested negative. To confirm these results, virus free greenhouse-grown sugarcane varieties Co997 and M1176/77, were mechanically inoculated with 10 sap extracts from 10 SCSMV-infected sugarcane leaf samples. Sap was also extracted from DAS-ELISA negative sugarcane leaves and used as negative control. For sap preparation, leaves were homogenized with a mortar in 2 mL of phosphate buffer 0.01 M pH 7.2 (ratio 1:10). Fifteen 4-week-old plants per variety were inoculated separately with each sap. All inoculated plants exhibited streak mosaic symptoms 13 days post-inoculation (fig. 1b), and the presence of SCSMV in the inoculated plants was confirmed by DAS-ELISA. Total RNA was extracted from four symptomatic leaf samples, one symptomless and one DAS-ELISA positive sample using TRIzol reagent (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized using M-MLV Reverse Transcriptase (Promega, Cat.No.M1705, Madison, WI, USA) following the manufacturer's instructions. A 690-nucleotide fragment of the CP gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) with primers SCSMV-F690 and SCSMV-R690 (Viswanathan et al. 2008). All primers were synthesized by Eurogentec (Seraing, Belgium). Aliquots of RT-PCR products (5 μl) were analyzed by electrophoresis on 1.2 % (w/v) agarose gel, stained with ethidium bromide and visualized on a UV transilluminator (Fig. 2). An amplification product of the expected size was obtained for all five symptomatic or DAS ELISA positive but not for the symptomless sample. Two RT-PCR products were sequenced and deposited in GenBank under accession Nos. LR594547 and LR594582. These partial CP gene sequences shared highest nucleotide identity with two isolates of SCSMV from India in GenBank: 91% with JN315855 and 90% with EF655859, thus confirming that SCSMV was occurring in sugarcane in Côte d'Ivoire. To our knowledge, this is the first report of natural infection of sugarcane by SCSMV in Africa. Streak mosaic is a serious threat to the entire sugar industry in West Africa and needs further investigations as it may affect sugarcane yields and impact local economies. Our findings further illustrate the need to develop virus-free germplasm for local, national, and international distribution of sugarcane. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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