Chemical Composition of an Anthelmintic Fraction of Pleurotus eryngii against Eggs and Infective Larvae (L3) of Haemonchus contortus .

Autor: Cruz-Arévalo J; El Colegio de la Frontera Sur, Carretera al Antiguo Aeropuerto km. 2.5 C.P, 30700 Tapachula, Chiapas, Mexico., Sánchez JE; El Colegio de la Frontera Sur, Carretera al Antiguo Aeropuerto km. 2.5 C.P, 30700 Tapachula, Chiapas, Mexico., González-Cortázar M; Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social (CIBIS-IMSS), Argentina No. 1. Col. Centro, Xochitepec, Morelos C.P. 62790, Mexico., Zamilpa A; Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social (CIBIS-IMSS), Argentina No. 1. Col. Centro, Xochitepec, Morelos C.P. 62790, Mexico., Andrade-Gallegos RH; El Colegio de la Frontera Sur, Carretera al Antiguo Aeropuerto km. 2.5 C.P, 30700 Tapachula, Chiapas, Mexico., Mendoza-de-Gives P; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, INIFAP, Km 11 Carretera Federal Cuernavaca-Cuautla, No. 8534, Col. Progreso, Morelos, Jiutepec, CP 62550, Mexico., Aguilar-Marcelino L; Centro Nacional de Investigación Disciplinaria en Salud Animal e Inocuidad, INIFAP, Km 11 Carretera Federal Cuernavaca-Cuautla, No. 8534, Col. Progreso, Morelos, Jiutepec, CP 62550, Mexico.
Jazyk: angličtina
Zdroj: BioMed research international [Biomed Res Int] 2020 Aug 06; Vol. 2020, pp. 4138950. Date of Electronic Publication: 2020 Aug 06 (Print Publication: 2020).
DOI: 10.1155/2020/4138950
Abstrakt: This study was aimed at evaluating the in vitro effect of the edible mushroom (EM) Pleurotus eryngii against the eggs and larvae (L3) of Haemonchus contortus . The evaluation included acetone (AE) and hydroalcoholic (HA) extracts of the following strains: ECS-1138, ECS-1156, ECS-1255, ECS-1258, ECS-1261, ECS-1282, and ECS-1292. The HA extract of the ECS-1255 strain showed the highest effect on mortality rates of L3 (18.83%) at 20  μ g/mL. After subjecting this HA extract to a normal phase chromatography column, five fractions were obtained; fraction F5 (100% MeOH) was the most effective against eggs, with hatching inhibition percentages of 88.77 and 91.87% at 20 and 40 mg/mL, respectively. Gas chromatography-mass spectrometry (GC-MS) subjected this fraction to an acetylation reaction to determine the content of the secondary metabolites. The GC-MS analysis showed that the F5 fraction was composed of trehalose CAS: 6138-23-4, polyols (L-iditol CAS: 488-45-9, galactitol CAS: 608-66-2, D-mannitol CAS: 69-65-8, D-glucitol CAS: 50-70-4, and myoinositol CAS: 87-89-8), adipic acid CAS: 124-04-9, stearic acid CAS: 57-11-4, squalene CAS: 111-02-4, and β -sitosterol CAS: 83-46-5.
Competing Interests: The authors declare that they have no conflicts of interest.
(Copyright © 2020 Julio Cruz-Arévalo et al.)
Databáze: MEDLINE
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