| Autor: |
Fan L; Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi., Gao W; Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi., Nguyen BV; Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi., Jefferson JR; Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi., Liu Y; Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi., Fan F; Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi., Roman RJ; Department of Pharmacology and Toxicology, University of Mississippi Medical Center, Jackson, Mississippi. |
| Abstrakt: |
Recently, we reported a mutation in γ-adducin (ADD3) was associated with an impaired myogenic response of the afferent arteriole and hypertension-induced chronic kidney disease (CKD) in fawn hooded hypertensive (FHH) rats. However, the mechanisms by which altered renal blood flow (RBF) autoregulation promotes hypertension-induced renal injury remain to be determined. The present study compared the time course of changes in renal hemodynamics and the progression of CKD during the development of DOCA-salt hypertension in FHH 1 BN congenic rats [wild-type (WT)] with an intact myogenic response versus FHH 1 BN Add3 KO ( Add3 KO) rats, which have impaired myogenic response. RBF was well autoregulated in WT rats but not in Add3 KO rats. Glomerular capillary pressure rose by 6 versus 14 mmHg in WT versus Add3 KO rats when blood pressure increased from 100 to 150 mmHg. After 1 wk of hypertension, glomerular filtration rate increased by 38% and glomerular nephrin expression decreased by 20% in Add3 KO rats. Neither were altered in WT rats. Proteinuria doubled in WT rats versus a sixfold increase in Add3 KO rats. The degree of renal injury was greater in Add3 KO than WT rats after 3 wk of hypertension. RBF, glomerular filtration rate, and glomerular capillary pressure were lower by 20%, 28%, and 19% in Add3 KO rats than in WT rats, which was associated with glomerular matrix expansion and loss of capillary filtration area. The results indicated that impaired RBF autoregulation and eutrophic remodeling of preglomerular arterioles increase the transmission of pressure to glomeruli, which induces podocyte loss and accelerates the progression of CKD in hypertensive Add3 KO rats. |
