Directed Evolution of CRISPR/Cas Systems for Precise Gene Editing.

Autor: Liu R; Renewable and Sustainable Energy Institute (RASEI), University of Colorado Boulder, Boulder, CO, USA. Electronic address: Rongming.liu@colorado.edu., Liang L; Renewable and Sustainable Energy Institute (RASEI), University of Colorado Boulder, Boulder, CO, USA., Freed EF; Renewable and Sustainable Energy Institute (RASEI), University of Colorado Boulder, Boulder, CO, USA., Gill RT; Renewable and Sustainable Energy Institute (RASEI), University of Colorado Boulder, Boulder, CO, USA; Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Lyngby, Denmark. Electronic address: rtg@biosustain.dtu.dk.
Jazyk: angličtina
Zdroj: Trends in biotechnology [Trends Biotechnol] 2021 Mar; Vol. 39 (3), pp. 262-273. Date of Electronic Publication: 2020 Aug 19.
DOI: 10.1016/j.tibtech.2020.07.005
Abstrakt: CRISPR technology is a universal tool for genome engineering that has revolutionized biotechnology. Recently identified unique CRISPR/Cas systems, as well as re-engineered Cas proteins, have rapidly expanded the functions and applications of CRISPR/Cas systems. The structures of Cas proteins are complex, containing multiple functional domains. These protein domains are evolutionarily conserved polypeptide units that generally show independent structural or functional properties. In this review, we propose using protein domains as a new way to classify protein engineering strategies for these proteins and discuss common ways to engineer key domains to modify the functions of CRISPR/Cas systems.
(Copyright © 2020 Elsevier Ltd. All rights reserved.)
Databáze: MEDLINE
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