Bed bugs, Cimex lectularius L., exhibiting metabolic and target site deltamethrin resistance are susceptible to plant essential oils.

Autor: Gaire S; Department of Entomology, Purdue University, West Lafayette, IN 47907, USA; Current address: Department of Entomology, University of Kentucky, Lexington, KY 40546, USA. Electronic address: sgaire@uky.edu., Lewis CD; Department of Biological Science, The University of Tulsa, Tulsa, OK 74104, USA., Booth W; Department of Biological Science, The University of Tulsa, Tulsa, OK 74104, USA., Scharf ME; Department of Entomology, Purdue University, West Lafayette, IN 47907, USA., Zheng W; School of Health Sciences, Purdue University, West Lafayette, IN 47907, USA., Ginzel MD; Department of Entomology, Purdue University, West Lafayette, IN 47907, USA., Gondhalekar AD; Department of Entomology, Purdue University, West Lafayette, IN 47907, USA. Electronic address: ameyag@purdue.edu.
Jazyk: angličtina
Zdroj: Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2020 Oct; Vol. 169, pp. 104667. Date of Electronic Publication: 2020 Jul 22.
DOI: 10.1016/j.pestbp.2020.104667
Abstrakt: Pyrethroid resistance has been a major hurdle limiting the effective control of bed bugs (Cimex lectularius L.). Alternative approaches that include the use of plant essential oils (EOs) have been proposed for effective management of bed bugs. However, EO resistance level comparisons between pyrethroid susceptible and resistant bed bug populations have not been previously conducted. The goal of this study was twofold: (i) determine deltamethrin resistance levels and associated resistance mechanisms in the field-collected Knoxville strain and (ii) quantify resistance levels of the Knoxville strain to five EOs (thyme, oregano, clove, geranium and coriander), their major insecticidal constituents (thymol, carvacrol, eugenol, geraniol and linalool) and an EO-based product (EcoRaider®). First, we found that the Knoxville strain was 72,893 and 291,626 fold more resistant to topically applied deltamethrin in comparison to the susceptible Harlan strain at the LD 25 and LD 50 lethal dose levels, respectively. Synergist bioassays and detoxification enzyme assays revealed significantly higher activity of cytochrome P450 and esterase enzymes in the resistant Knoxville strain. Further, Sanger sequencing revealed the presence of the L925I mutation in the voltage-sensitive sodium channel α subunit gene. The Knoxville strain that possesses both enzymatic and target site deltamethrin resistance, however, did not show any resistance to EOs, their major insecticidal constituents and EcoRaider® in topical bioassays (resistance ratio of ~1). In conclusion, this study demonstrated that a deltamethrin-resistant strain of bed bugs is susceptible to EOs and their insecticidal constituents.
Competing Interests: Declaration of Competing Interest The authors declare no competing interests.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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