Method for detection of mtDNA damages for evaluating of pesticides toxicity for bumblebees (Bombus terrestris L.).

Autor: Syromyatnikov MY; Voronezh State University, Voronezh, University sq. 1, Voronezh 394018, Russia; Voronezh State University of Engineering Technologies, Revolution Av. 19, Voronezh 394036, Russia. Electronic address: syromyatnikov@bio.vsu.ru., Gureev AP; Voronezh State University, Voronezh, University sq. 1, Voronezh 394018, Russia., Starkova NN; Maritime College, State University of New York, 6 Pennyfield Avenue Throggs Neck, NY 10465, USA., Savinkova OV; Voronezh State University, Voronezh, University sq. 1, Voronezh 394018, Russia., Starkov AA; Weill Medical College Cornell University, 525 E 68th street, A501, New York, NY 10065, USA., Lopatin AV; Voronezh State University, Voronezh, University sq. 1, Voronezh 394018, Russia., Popov VN; Voronezh State University, Voronezh, University sq. 1, Voronezh 394018, Russia; Voronezh State University of Engineering Technologies, Revolution Av. 19, Voronezh 394036, Russia.
Jazyk: angličtina
Zdroj: Pesticide biochemistry and physiology [Pestic Biochem Physiol] 2020 Oct; Vol. 169, pp. 104675. Date of Electronic Publication: 2020 Aug 05.
DOI: 10.1016/j.pestbp.2020.104675
Abstrakt: Bumblebees are important for crop pollination. Currently, the number of pollinators is decreasing worldwide, which is attributed mostly to the widespread use of pesticides. The aim of this work was to develop a method for assessing the genotoxicity of pesticides for the Bombus terrestris L. bumblebee using long-range PCR of mitochondrial DNA fragments. We have developed a panel of primers and assessed the genotoxicity of the following pesticides: imidacloprid, rotenone, deltamethrin, difenocanozole, malathion, metribuzin, penconazole, esfenvalerate, and dithianon. All pesticides (except imidacloprid) inhibited mitochondrial respiration fueled by pyruvate + malate; the strongest effect was observed for rotenone and difenocanozole. Three pesticides (dithianon, rotenone, and difenocanozole) affected the rate of H 2 O 2 production. To study the pesticide-induced DNA damage in vitro and in vivo, we used three different mtDNA. The mtDNA damage was observed for all studied pesticides. Most of the studied pesticides caused significant damage to mtDNA in vitro and in vivo when ingested. Our results indicate that all tested pesticides, including herbicides and fungicides, can have a toxic effect on pollinators. However, the extent of pesticide-induced mtDNA damage in the flight muscles was significantly less upon the contact compared to the oral administration.
(Copyright © 2020 Elsevier Inc. All rights reserved.)
Databáze: MEDLINE
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