Chemical profile and gastroprotective effect of Jatropha elliptica (Pohl) Oken roots.

Autor: Coelho IP; Universidade Federal de Mato Grosso do Sul, Faculdade de Medicina, Campo Grande, Mato Grosso do Sul, Brazil., Santos LBBD; Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, Mato Grosso do Sul, Brazil., Kato Junior WH; Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, Mato Grosso do Sul, Brazil., Corsino J; Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, Mato Grosso do Sul, Brazil., Cordeiro KW; Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, Mato Grosso do Sul, Brazil., Boeing T; Universidade do Vale do Itajaí, Núcleo de Investigações Químico-Farmacêuticas, Itajaí, Santa Catarina, Brazil., Coelho JM; Universidade Federal de Mato Grosso do Sul, Faculdade de Medicina, Campo Grande, Mato Grosso do Sul, Brazil., Garcez FR; Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, Mato Grosso do Sul, Brazil., Garcez WS; Universidade Federal de Mato Grosso do Sul, Faculdade de Medicina, Campo Grande, Mato Grosso do Sul, Brazil; Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, Mato Grosso do Sul, Brazil., de Andrade SF; Universidade do Vale do Itajaí, Núcleo de Investigações Químico-Farmacêuticas, Itajaí, Santa Catarina, Brazil; Universidade Lusófona CBIOS, Research Center for Biosciences and Health Technologies, Av. Campo Grande, 376, 1749-024 Lisboa, Portugal., Figueiredo PO; Universidade Federal de Mato Grosso do Sul, Instituto de Química, Campo Grande, Mato Grosso do Sul, Brazil. Electronic address: patricia.figueiredo@ufms.br.
Jazyk: angličtina
Zdroj: Fitoterapia [Fitoterapia] 2020 Oct; Vol. 146, pp. 104707. Date of Electronic Publication: 2020 Aug 19.
DOI: 10.1016/j.fitote.2020.104707
Abstrakt: Jatropha elliptica (Pohl) Oken (Euphorbiaceae) roots are used in folk medicine to treat gastric ulcers. The purpose of this work was to evaluate the gastroprotective activity of ethanol extract (JER) and hexane fraction (ERH) of J. elliptica roots in mice, as well as to analyze the acute toxicity of the extract and identify the potential active compounds. No signs of toxicity were observed in JER. In both acidified ethanol and indometacin-induced gastric ulcer models, all doses tested of JER and ERH significantly reduced gastric lesions. Dereplication of JER was performed by HPLC-DAD-ESI-MS/MS and resulted in the annotation of compounds fraxetin, propacin, jatrophone and jatropholones A and B. GC-MS analysis of ERH revealed the diterpenes jatrophone, jatropholone A and jatropholone B as the major components. The chemical study of this fraction has led to the isolation of these compounds, in addition to the sequiterpene cyperenoic acid and the diterpene 2β-hydroxyjatrophone, both reported for the first time in J. elliptica. The isolated compounds were tested against L929 cells and only cyperenoic acid and the mixture of jatropholones A and B did not show toxicity, being then selected as good candidates for bioassays using acidified ethanol-induced gastric ulcer model. Cyperenoic acid significantly decreased gastric lesions and preserved gastric mucus layer. The mixture of jatropholones A and B caused a smaller reduction of gastric lesions, without preservation of the gastric mucus layer. The study showed that J. elliptica roots present gastroprotective activity in mice, without causing acute toxic effects. The activity is related, at least in part, to the occurrence of terpenes, mainly the sesquiterpene cyperenoic acid.
Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2020 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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