Effects of Acute Subdural Hematoma-Induced Brain Injury On Energy Metabolism in Peripheral Blood Mononuclear Cells.

Autor:	Zink F; Institue of Anesthesiological Pathophysiology and Process Development, Ulm University Hospital, Ulm, Germany., Vogt J; Institue of Anesthesiological Pathophysiology and Process Development, Ulm University Hospital, Ulm, Germany., Wachter U; Institue of Anesthesiological Pathophysiology and Process Development, Ulm University Hospital, Ulm, Germany., Hartert J; Department of Anesthesiology, Ulm University Hospital, Ulm, Germany., Horchler M; Institue of Anesthesiological Pathophysiology and Process Development, Ulm University Hospital, Ulm, Germany., Zhang X; Institue of Anesthesiological Pathophysiology and Process Development, Ulm University Hospital, Ulm, Germany., Hezel F; Institue of Anesthesiological Pathophysiology and Process Development, Ulm University Hospital, Ulm, Germany., Kapapa T; Department of Neurosurgery, Ulm University Hospital, Ulm, Germany., Datzmann T; Department of Anesthesiology, Ulm University Hospital, Ulm, Germany., Hoffmann A; Institue of Anesthesiological Pathophysiology and Process Development, Ulm University Hospital, Ulm, Germany., Wepler M; Department of Anesthesiology, Ulm University Hospital, Ulm, Germany., Calzia E; Institue of Anesthesiological Pathophysiology and Process Development, Ulm University Hospital, Ulm, Germany., Radermacher P; Institue of Anesthesiological Pathophysiology and Process Development, Ulm University Hospital, Ulm, Germany., Hartmann C; Institue of Anesthesiological Pathophysiology and Process Development, Ulm University Hospital, Ulm, Germany.; Department of Anesthesiology, Ulm University Hospital, Ulm, Germany.
Jazyk:	angličtina
Zdroj:	Shock (Augusta, Ga.) [Shock] 2021 Mar 01; Vol. 55 (3), pp. 407-417.
DOI:	10.1097/SHK.0000000000001642
Abstrakt:	Abstract: In activated immune cells, differentiation and function are determined by cell type-specific modifications of metabolic patterns. After traumatic brain injury both immune cell activation and suppression were reported. Therefore, we sought to explore immune cell energy metabolism in a long-term, resuscitated porcine model of acute subdural hematoma (ASDH)-induced acute brain injury devoid of impaired systemic hemodynamics and oxygen transport.Before and up to 50 h after induction of ASDH, peripheral blood mononuclear cells (PBMCs) were separated by density gradient centrifugation, and cell metabolism was analyzed using high-resolution respirometry for mitochondrial respiration and electron spin resonance for reactive oxygen species production. After incubation with stable isotope-labeled 1,2-13C2-glucose or 13C5-glutamine, distinct labeling patterns of intermediates of glycolysis or tricarboxylic acid (TCA) cycle and 13CO2 production were measured by gas chromatography-mass spectroscopy. Principal component analysis was followed by a varimax rotation on the covariance across all measured variables and all measured time points.After ASDH induction, average PBMC metabolic activity remained unaffected, possibly because strict adherence to intensive care unit guidelines limited trauma to ASDH induction without any change in parameters of systemic hemodynamics, oxygen transport, and whole-body metabolism. Despite decreased glycolytic activity fueling the TCA cycle, the principal component analysis indicated a cell type-specific activation pattern with biosynthetic and proliferative characteristics. Competing Interests: The authors report no conflicts of interest. (Copyright © 2020 by the Shock Society.)
Databáze:	MEDLINE
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