| Autor: |
Chabanol E; Microbiota of Insect Vectors Group, Institut Pasteur de Guyane, Cayenne 97306, French Guiana.; Ecole Doctorale Numéro 587, Diversités, Santé, et Développement en Amazonie, Université de Guyane, Cayenne 97337, French Guiana.; Tropical Biome and Immunophysiopathology, Université de Guyane, Cayenne 97300, French Guiana., Behrends V; Health Sciences Research Centre, University of Roehampton, London SW15 4JD, UK., Prévot G; Tropical Biome and Immunophysiopathology, Université de Guyane, Cayenne 97300, French Guiana.; CNRS, INSERM, Centre d'Infection et d'Immunité de Lille, Institut Pasteur de Lille, Université de Lille, 59000 Lille, France., Christophides GK; Department of Life Sciences, Imperial College London, London SW7 2BU, UK., Gendrin M; Microbiota of Insect Vectors Group, Institut Pasteur de Guyane, Cayenne 97306, French Guiana.; Department of Life Sciences, Imperial College London, London SW7 2BU, UK.; Department of Parasites and Insect Vectors, Institut Pasteur, 75015 Paris, France. |
| Abstrakt: |
The mosquito microbiota reduces the vector competence of Anopheles to Plasmodium and affects host fitness; it is therefore considered as a potential target to reduce malaria transmission. While immune induction, secretion of antimicrobials and metabolic competition are three typical mechanisms of microbiota-mediated protection against invasive pathogens in mammals, the involvement of metabolic competition or mutualism in mosquito-microbiota and microbiota- Plasmodium interactions has not been investigated. Here, we describe a metabolome analysis of the midgut of Anopheles coluzzii provided with a sugar-meal or a non-infectious blood-meal, under conventional or antibiotic-treated conditions. We observed that the antibiotic treatment affects the tricarboxylic acid cycle and nitrogen metabolism, notably resulting in decreased abundance of free amino acids. Linking our results with published data, we identified pathways which may participate in microbiota- Plasmodium interactions via metabolic interactions or immune modulation and thus would be interesting candidates for future functional studies. |
