| Autor: |
Abdelmanova AS; L.K. Ernst Federal Science Center for Animal Husbandry, 60, pos. Dubrovitsy, 142132 Podolsk, Russia., Kharzinova VR; L.K. Ernst Federal Science Center for Animal Husbandry, 60, pos. Dubrovitsy, 142132 Podolsk, Russia., Volkova VV; L.K. Ernst Federal Science Center for Animal Husbandry, 60, pos. Dubrovitsy, 142132 Podolsk, Russia., Mishina AI; L.K. Ernst Federal Science Center for Animal Husbandry, 60, pos. Dubrovitsy, 142132 Podolsk, Russia., Dotsev AV; L.K. Ernst Federal Science Center for Animal Husbandry, 60, pos. Dubrovitsy, 142132 Podolsk, Russia., Sermyagin AA; L.K. Ernst Federal Science Center for Animal Husbandry, 60, pos. Dubrovitsy, 142132 Podolsk, Russia., Boronetskaya OI; L.K. Ernst Federal Science Center for Animal Husbandry, 60, pos. Dubrovitsy, 142132 Podolsk, Russia.; Timiryazev Russian State Agrarian University-Moscow Agrarian Academy, 49, ul. Timiryazevskaya, 127550 Moscow, Russia., Petrikeeva LV; L.K. Ernst Federal Science Center for Animal Husbandry, 60, pos. Dubrovitsy, 142132 Podolsk, Russia.; Timiryazev Russian State Agrarian University-Moscow Agrarian Academy, 49, ul. Timiryazevskaya, 127550 Moscow, Russia., Chinarov RY; L.K. Ernst Federal Science Center for Animal Husbandry, 60, pos. Dubrovitsy, 142132 Podolsk, Russia., Brem G; L.K. Ernst Federal Science Center for Animal Husbandry, 60, pos. Dubrovitsy, 142132 Podolsk, Russia.; Institut für Tierzucht und Genetik, University of Veterinary Medicine (VMU), Veterinärplatz, A-1210 Vienna, Austria., Zinovieva NA; L.K. Ernst Federal Science Center for Animal Husbandry, 60, pos. Dubrovitsy, 142132 Podolsk, Russia. |
| Abstrakt: |
Analysis of ancient and historical DNA has great potential to trace the genetic diversity of local cattle populations during their centuries-long development. Forty-nine specimens representing five cattle breeds (Kholmogor, Yaroslavl, Great Russian, Novgorod, and Holland), dated from the end of the 19th century to the first half of the 20th century, were genotyped for nine polymorphic microsatellite loci. Using a multiple-tube approach, we determined the consensus genotypes of all samples/loci analysed. Amplification errors, including allelic drop-out (ADO) and false alleles (FA), occurred with an average frequency of 2.35% and 0.79%, respectively. A significant effect of allelic length on ADO rate ( r 2 = 0.620, p = 0.05) was shown. We did not observe significant differences in genetic diversity among historical samples and modern representatives of Kholmogor and Yaroslavl breeds. The unbiased expected heterozygosity values were 0.726-0.774 and 0.708-0.739; the allelic richness values were 2.716-2.893 and 2.661-2.758 for the historical and modern samples, respectively. Analyses of F ST and Jost's D genetic distances, and the results of STRUCTURE clustering, showed the maintenance of a part of historical components in the modern populations of Kholmogor and Yaroslavl cattle. Our study contributes to the conservation of biodiversity in the local Russian genetic resources of cattle. |
