Predictive modeling of complex ABO glycan phenotypes by lectin microarrays.

Autor:	Anani WQ; Medical Sciences Institute, Versiti, Milwaukee, WI.; Department of Pathology, Medical College of Wisconsin, Milwaukee, WI.; Translational Glycomics Center., Ashwood HE; Translational Glycomics Center.; Blood Research Institute, and., Schmidt A; Translational Glycomics Center., Burns RT; Translational Glycomics Center., Denomme GA; Translational Glycomics Center.; Diagnostic Laboratories, Versiti, Milwaukee, WI; and., Hoffmeister KM; Translational Glycomics Center.; Blood Research Institute, and.; Department of Biochemistry and Medicine, Medical College of Wisconsin, Milwaukee, WI.
Jazyk:	angličtina
Zdroj:	Blood advances [Blood Adv] 2020 Aug 25; Vol. 4 (16), pp. 3960-3970.
DOI:	10.1182/bloodadvances.2020002051
Abstrakt:	Serological classification of individuals as A, B, O, or AB is a mainstay of blood banking. ABO blood groups or ABH antigens, in addition to other surface glycans, act as unique red blood cell (RBC) signatures and direct immune responses. ABO subgroups present as weakened, mixed field, or unexpected reactivity with serological reagents, but specific designations remain complex. Lectins detect glycan motifs with some recognizing ABH antigens. We evaluated a 45-probe lectin microarray to rapidly analyze ABO blood groups and associated unique glycan signatures within complex biological samples on RBC surface glycoproteins. RBC membrane glycoproteins were prepared from donor RBCs, n = 20 for each blood group. ABO blood group was distinguishable by lectin array, including variations in ABH antigen expression not observed with serology. Principal component analysis highlighted broad ABO blood group clusters with unexpected high and low antigen expression and variations were confirmed with ABH antibody immunoblotting. Using a subset of lectins provided an accurate method to predict an ABO serological phenotype. Lectin microarray highlighted the importance of ABO localization on glycoproteins and glycolipids and pointed to increased glycocalyx complexity associated with the expression of A and B antigens including high mannose and branched polylactosamine. Thus, lectins identified subtle surface ABO blood group glycoprotein density variations not detected by routine serological methods. Transfusion services observe alterations in ABH expression during malignancy, and ABO incompatible solid organ transplantation is not without risk of rejection. The presented methods may identify subtle but clinically significant ABO blood group differences for transfusion and transplantation. (© 2020 by The American Society of Hematology.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu