More cell culture passaged Camelpox virus sequences found resembling those of vaccinia virus.
| Autor: | Khalafalla AI; Veterinary Laboratories Division, Animal Health Sector, Abu Dhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, UAE.; Department of Microbiology, Faculty of Veterinary Medicine, University of Khartoum, Shambat, Khartoum North Sudan., Al Hosani MA; Veterinary Laboratories Division, Animal Health Sector, Abu Dhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, UAE., Ishag HZA; Veterinary Laboratories Division, Animal Health Sector, Abu Dhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, UAE., Al Muhairi SS; Veterinary Laboratories Division, Animal Health Sector, Abu Dhabi Agriculture and Food Safety Authority (ADAFSA), Abu Dhabi, UAE. |
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| Jazyk: | angličtina |
| Zdroj: | Open veterinary journal [Open Vet J] 2020 Aug; Vol. 10 (2), pp. 144-156. Date of Electronic Publication: 2020 Apr 23. |
| DOI: | 10.4314/ovj.v10i2.4 |
| Abstrakt: | Background: Camelpox is the most infectious and economically important disease of camelids that causes significant morbidity and mortality rates. Several live attenuated vaccines against Camelpox virus (CMLV) are produced worldwide by passaging field isolates in cell culture. Sequence of a high passage Saudi isolate of CMLV was previously found closely resembled Vaccinia virus (VACV). Aim: To determine whether other high cell culture passage CMLV isolates are genetically resemble VACV and further to explore the possible mechanism of the resemblance. Methods: We performed polymerase chain reaction and DNA sequence analysis of A-type inclusion body protein (ATIP), L1R, and open reading frame (ORF) 185 genes on different cell culture passage levels of a field isolate, two high passage vaccines, wild-type, and reference strains of CMLV. Results: We demonstrate that additional two high passage attenuated vaccine candidate from Sudan and UAE likewise contain sequences resembling VACV more than CMLV. Furthermore, sequence analysis of the ATIP gene of selected virus passages in cell culture revealed that the shift to VACV-like occurred between passage 11 and 20 and up to the 10th passage the genome still resembles wild-type virus. This observation was further confirmed by recombination analysis which indicated recombination events at ATIP and ORF185 genes occurred at higher passages. Conclusion: We confirmed that the cell culture passage CMLV turns to resemble VACV after cell culture passage and concluded that the resemblance may not be a result of contamination or misidentification as previously thought but could be due to recombination events that occurred during the passage process. Competing Interests: The authors declare that they have no conflict of interest. |
| Databáze: | MEDLINE |
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