Estrogen Regulates Local Cysteine Metabolism in Mouse Myometrium.

Autor: Guerra DD; Department of Biology , University of Louisville , 2301 South 3rd Street, Louisville, Kentucky, 40292, USA., Bok R; Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Aurora, CO, 80045, USA., Breen K; Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Aurora, CO, 80045, USA., Vyas V; Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Aurora, CO, 80045, USA., Jiang H; Section of Clinical Genetics and Metabolism, Department of Pediatrics, University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Aurora, CO, 80045, USA., MacLean KN; Section of Clinical Genetics and Metabolism, Department of Pediatrics, University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Aurora, CO, 80045, USA., Hurt KJ; Division of Reproductive Sciences, Department of Obstetrics and Gynecology, University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Aurora, CO, 80045, USA. k.joseph.hurt@cuanschutz.edu.; Division of Maternal Fetal Medicine, Department of Obstetrics and Gynecology, University of Colorado Anschutz Medical Campus, 12700 East 19th Avenue, Mail Stop 8613, Aurora, CO, 80045, USA. k.joseph.hurt@cuanschutz.edu.
Jazyk: angličtina
Zdroj: Reproductive sciences (Thousand Oaks, Calif.) [Reprod Sci] 2021 Jan; Vol. 28 (1), pp. 79-90. Date of Electronic Publication: 2020 Aug 20.
DOI: 10.1007/s43032-020-00284-6
Abstrakt: Sulfur amino acid metabolism influences reproductive physiology, and transsulfuration in particular may be critical for normal cellular function. The sex hormone estrogen (E 2 ) modulates gene expression and redox balance in some tissues by inducing the transsulfuration enzymes cystathionine β-synthase (CBS) and cystathionine γ-lyase (CSE). The role of sex hormones in sulfur amino acid metabolism by uterine smooth muscle is not known. Here, we show that CBS and CSE proteins increase in the mouse myometrium during estrus and diestrus, respectively, suggesting that E 2 reciprocally regulates myometrial CBS and CSE expression. In ovariectomized mice, exogenous E 2 upregulates CBS and downregulates CSE levels. E 2 promotes CBS mRNA and protein expression but attenuates CSE protein expression without affecting CSE mRNA. This pattern of E 2 -stimulated changes in transsulfuration enzyme expression is specific to the uterine smooth muscle. E 2 does not change vaginal or cervical expression of CBS or CSE significantly, and E 2 decreases expression of CSE in the liver without affecting CBS. E 2 also downregulates myometrial cysteinesulfinic acid decarboxylase (CSAD) and decreases myometrial biochemical synthesis of the gaso-transmitter hydrogen sulfide (H 2 S). These findings suggest that myometrial sulfur amino acid metabolism may regulate uterine redox homeostasis, with implications for the source and metabolism of myometrial cysteine in high E 2 states such as estrus and pregnancy.
Databáze: MEDLINE
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