4C-Seq: Interrogating Chromatin Looping with Circular Chromosome Conformation Capture.
| Autor: | Karasu N; Institute of Genetics and Molecular and Cellular Biology (IGBMC), Illkirch, France.; CNRS UMR7104, Illkirch, France.; INSERM U1258, Illkirch, France.; University of Strasbourg, Illkirch, France., Sexton T; Institute of Genetics and Molecular and Cellular Biology (IGBMC), Illkirch, France. sexton@igbmc.fr.; CNRS UMR7104, Illkirch, France. sexton@igbmc.fr.; INSERM U1258, Illkirch, France. sexton@igbmc.fr.; University of Strasbourg, Illkirch, France. sexton@igbmc.fr. |
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| Jazyk: | angličtina |
| Zdroj: | Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2021; Vol. 2157, pp. 19-34. |
| DOI: | 10.1007/978-1-0716-0664-3_3 |
| Abstrakt: | Chromosome conformation capture and its variants have allowed chromatin topology to be interrogated at a superior resolution and throughput than by microscopic methods. Among the method derivatives, 4C-seq (circular chromosome conformation capture, coupled to high-throughput sequencing) is a versatile, cost-effective means of assessing all chromatin interactions with a specific genomic region of interest, making it particularly suitable for interrogating chromatin looping events. We present the principles and procedures for designing and implementing successful 4C-seq experiments. |
| Databáze: | MEDLINE |
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