Rational Design of Suprastat: A Novel Selective Histone Deacetylase 6 Inhibitor with the Ability to Potentiate Immunotherapy in Melanoma Models.

Autor: Noonepalle S; Department of Biochemistry and Molecular Medicine, The George Washington University, Washington, District of Columbia 20052, United States., Shen S; Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois 60612, United States., Ptáček J; Laboratory of Structural Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, Vestec 252 50, Czech Republic., Tavares MT; Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois 60612, United States., Zhang G; Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, Chicago, Illinois 60612, United States., Stránský J; Centre of Molecular Structure, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, Vestec 252 50, Czech Republic., Pavlíček J; Centre of Molecular Structure, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, Vestec 252 50, Czech Republic., Ferreira GM; Department of Pharmacy, School of Pharmaceutical Sciences, University of São Paulo, São Paulo, SP 05508-000, Brazil., Hadley M; Department of Biochemistry and Molecular Medicine, The George Washington University, Washington, District of Columbia 20052, United States., Pelaez G; Department of Biochemistry and Molecular Medicine, The George Washington University, Washington, District of Columbia 20052, United States., Bařinka C; Laboratory of Structural Biology, Institute of Biotechnology of the Czech Academy of Sciences, BIOCEV, Prumyslova 595, Vestec 252 50, Czech Republic., Kozikowski AP; Bright Minds Biosciences, Toronto, ON M5H 3V9, Canada., Villagra A; Department of Biochemistry and Molecular Medicine, The George Washington University, Washington, District of Columbia 20052, United States.
Jazyk: angličtina
Zdroj: Journal of medicinal chemistry [J Med Chem] 2020 Sep 24; Vol. 63 (18), pp. 10246-10262. Date of Electronic Publication: 2020 Sep 02.
DOI: 10.1021/acs.jmedchem.0c00567
Abstrakt: Selective inhibition of histone deacetylase 6 (HDAC6) is being recognized as a therapeutic approach for cancers. In this study, we designed a new HDAC6 inhibitor, named Suprastat, using in silico simulations. X-ray crystallography and molecular dynamics simulations provide strong evidence to support the notion that the aminomethyl and hydroxyl groups in the capping group of Suprastat establish significant hydrogen bond interactions, either direct or water-mediated, with residues D460, N530, and S531, which play a vital role in regulating the deacetylase function of the enzyme and which are absent in other isoforms. In vitro characterization of Suprastat demonstrates subnanomolar HDAC6 inhibitory potency and a hundred- to a thousand-fold HDAC6 selectivity over the other HDAC isoforms. In vivo studies reveal that a combination of Suprastat and anti-PD1 immunotherapy enhances antitumor immune response, mediated by a decrease of protumoral M2 macrophages and increased infiltration of antitumor CD8+ effector and memory T-cells.
Databáze: MEDLINE
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