The sugarcane ShMYB78 transcription factor activates suberin biosynthesis in Nicotiana benthamiana.

Autor: Figueiredo R; Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, 13083-862, Brazil. raquelfigdo@gmail.com.; Department of Biology, Faculdade de Ciências, Universidade Do Porto, Rua Do Campo Alegre S/N, 4169-007, Porto, Portugal. raquelfigdo@gmail.com., Portilla Llerena JP; Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, 13083-862, Brazil., Kiyota E; Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, 13083-862, Brazil., Ferreira SS; Department of Botany, Institute of Biosciences, University of São Paulo, São Paulo, 05508-090, Brazil., Cardeli BR; Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, 13083-862, Brazil., de Souza SCR; Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, 13083-862, Brazil.; Department of Botany, Federal University of São Carlos, PO Box 676, São Carlos, São Paulo, 13565-905, Brazil., Dos Santos Brito M; Institute of Science and Technology, Federal University of São Paulo, Campus São José dos Campos, São José dos Campos, 12231-280, Brazil., Sodek L; Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, 13083-862, Brazil., Cesarino I; Department of Botany, Institute of Biosciences, University of São Paulo, São Paulo, 05508-090, Brazil., Mazzafera P; Department of Plant Biology, Institute of Biology, State University of Campinas, Campinas, 13083-862, Brazil.; Department of Crop Science, College of Agriculture Luiz de Queiroz, University of São Paulo, Piracicaba, 13418-900, Brazil.
Jazyk: angličtina
Zdroj: Plant molecular biology [Plant Mol Biol] 2020 Nov; Vol. 104 (4-5), pp. 411-427. Date of Electronic Publication: 2020 Aug 19.
DOI: 10.1007/s11103-020-01048-1
Abstrakt: Key Message: A sugarcane MYB present in the culm induces suberin biosynthesis and is involved both with fatty acid and phenolics metabolism. Few transcription factors have been described as regulators of cell wall polymers deposition in C4 grasses. Particularly, regulation of suberin biosynthesis in this group of plants remains poorly understood. Here, we showed that the sugarcane MYB transcription factor ShMYB78 is an activator of suberin biosynthesis and deposition. ShMYB78 was identified upon screening genes whose expression was upregulated in sugarcane internodes undergoing suberization during culm development or triggered by wounding. Agrobacterium-mediated transient expression of ShMYB78 in Nicotiana benthamiana leaves induced the ectopic deposition of suberin and its aliphatic and aromatic monomers. Further, the expression of suberin-related genes was induced by ShMYB78 heterologous expression in Nicotiana benthamiana leaves. ShMYB78 was shown to be a nuclear protein based on its presence in sugarcane internode nuclear protein extracts, and protoplast transactivation assays demonstrated that ShMYB78 activates the promoters of the sugarcane suberin biosynthetic genes β-ketoacyl-CoA synthase (ShKCS20) and caffeic acid-O-methyltransferase (ShCOMT). Our results suggest that ShMYB78 may be involved in the transcriptional regulation of suberin deposition, from fatty acid metabolism to phenylpropanoid biosynthesis, in sugarcane internodes.
Databáze: MEDLINE
Externí odkaz: